文章：

长链非编码RNA在复杂疾病中的非RNA依赖性调控功能

RNA-Independent Regulatory Functions of lncRNA in Complex Disease

原文发布日期：31 July 2024

DOI: 10.3390/cancers16152728

类型: Article

开放获取: 是

英文摘要：

During the metagenomics era, high-throughput sequencing efforts both in mice and humans indicate that non-coding RNAs (ncRNAs) constitute a significant fraction of the transcribed genome. During the past decades, the regulatory role of these non-coding transcripts along with their interactions with other molecules have been extensively characterized. However, the study of long non-coding RNAs (lncRNAs), an ncRNA regulatory class with transcript lengths that exceed 200 nucleotides, revealed that certain non-coding transcripts are transcriptional “by-products”, while their loci exert their downstream regulatory functions through RNA-independent mechanisms. Such mechanisms include, but are not limited to, chromatin interactions and complex promoter-enhancer competition schemes that involve the underlying ncRNA locus with or without its nascent transcription, mediating significant or even exclusive roles in the regulation of downstream target genes in mammals. Interestingly, such RNA-independent mechanisms often drive pathological manifestations, including oncogenesis. In this review, we summarize selective examples of lncRNAs that regulate target genes independently of their produced transcripts.

摘要翻译： 

在宏基因组学时代，针对小鼠和人类的高通量测序研究表明，非编码RNA（ncRNAs）构成了转录基因组的重要组成部分。过去几十年来，这些非编码转录物的调控作用及其与其他分子的相互作用已得到广泛研究。然而，对长度超过200个核苷酸的非编码RNA调控类别——长链非编码RNA（lncRNAs）的研究揭示，某些非编码转录物是转录的“副产物”，而其基因座通过不依赖于RNA的机制发挥下游调控功能。这些机制包括但不限于染色质相互作用和复杂的启动子-增强子竞争模式，这些模式涉及基础的非编码RNA基因座（无论其是否进行新生转录），在哺乳动物下游靶基因的调控中发挥重要甚至主导作用。有趣的是，这种不依赖于RNA的机制常常驱动病理表现，包括肿瘤发生。在本综述中，我们总结了不依赖于其转录产物而调控靶基因的lncRNAs的选择性案例。

原文链接：

RNA-Independent Regulatory Functions of lncRNA in Complex Disease