Introduction: Decellularized extracellular matrix (ECM) bioscaffolds have emerged as a promising three-dimensional (3D) model, but so far there are no data concerning their use in radiobiological studies. Material and Methods: We seeded two well-known radioresistant cell lines (HMV-II and PANC-1) in decellularized porcine liver-derived scaffolds and irradiated them with both high- (Carbon Ions) and low- (Photons) Linear Energy Transfer (LET) radiation in order to test whether a natural 3D-bioscaffold might be a useful tool for radiobiological research and to achieve an evaluation that could be as near as possible to what happens in vivo. Results: Biological scaffolds provided a favorable 3D environment for cell proliferation and expansion. Cells did not show signs of dedifferentiation and retained their distinct phenotype coherently with their anatomopathological and clinical behaviors. The radiobiological response to high LET was higher for HMV-II and PANC-1 compared to the low LET. In particular, Carbon Ions reduced the melanogenesis in HMV-II and induced more cytopathic effects and the substantial cell deterioration of both cell lines compared to photons. Conclusions: In addition to offering a suitable 3D model for radiobiological research and an appropriate setting for preclinical oncological analysis, we can attest that bioscaffolds seemed cost-effective due to their ease of use, low maintenance requirements, and lack of complex technology
引言：脱细胞细胞外基质（ECM）生物支架已成为一种有前景的三维（3D）模型，但迄今为止尚无关于其在放射生物学研究中应用的数据。材料与方法：我们将两种已知的放射抗性细胞系（HMV-II和PANC-1）接种于脱细胞猪肝来源的支架中，并分别采用高线性能量转移（LET）辐射（碳离子）和低LET辐射（光子）进行照射，旨在验证天然3D生物支架是否可作为放射生物学研究的有效工具，并尽可能模拟体内环境进行评估。结果：生物支架为细胞增殖和扩增提供了有利的3D环境。细胞未出现去分化迹象，并保持与其解剖病理学和临床行为一致的表型特征。相较于低LET辐射，HMV-II和PANC-1对高LET辐射的放射生物学反应更为显著。具体而言，与光子辐射相比，碳离子辐射降低了HMV-II细胞的黑色素生成，并对两种细胞系均诱导了更强的细胞病变效应及显著的细胞损伤。结论：除了为放射生物学研究提供合适的3D模型及临床前肿瘤学分析的适宜环境外，我们可以证实生物支架因其操作简便、维护需求低且无需复杂技术，具有显著的成本效益优势。
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