Globally, an increasing prevalence of colorectal cancer (CRC) prompts a need for the development of new methods for early tumor detection. MicroRNAs (also referred to as miRNAs) are short non-coding RNA molecules that play a pivotal role in the regulation of gene expression. MiRNAs are effectively transferred to extracellular vesicle (EVs) membrane sacs commonly released by cells. Our study aimed to examine the expression of miRNAs in four CRC cell lines and EVs derived from them (tumor EVs) in comparison to the normal colon epithelium cell line and its EVs. EVs were isolated by ultracentrifugation from the culture supernatant of SW480, SW620, SW1116, HCT116 and normal CCD841CoN cell lines and characterized according to the MISEV2023 guidelines. MiRNAs were analyzed by small RNA sequencing and validated by quantitative PCR. The performed analysis revealed 22 common miRNAs highly expressed in CRC cell lines and effectively transferred to tumor EVs, including miR-9-5p, miR-182-5p, miR-196b-5p, miR-200b-5p, miR-200c-3p, miR-425-5p and miR-429, which are associated with development, proliferation, invasion and migration of colorectal cancer cells, as well as in vesicle maturation and transport-associated pathways. In parallel, normal cells expressed miRNAs, such as miR-369 and miR-143, which play a role in proinflammatory response and tumor suppression. The analysis of selected miRNAs in plasma-derived EVs and tumor samples from CRC patients showed the similarity of miRNA expression profile between the patients’ samples and CRC cell lines. Moreover, miR-182-5p, miR-196-5p, miR-425-5p and miR-429 were detected in several EV samples isolated from patients’ plasma. Our results suggest that miR-182-5p, miR-196b-5p and miR-429 are differentially expressed between EVs from CRC patients and healthy donors, which might have clinical implications.
全球范围内结直肠癌发病率的不断上升，亟需开发新的早期肿瘤检测方法。微小RNA（亦称miRNAs）是一类短链非编码RNA分子，在基因表达调控中发挥关键作用。miRNAs可有效转运至细胞通常释放的细胞外囊泡膜囊中。本研究旨在比较四种结直肠癌细胞系及其来源的细胞外囊泡（肿瘤囊泡）与正常结肠上皮细胞系及其囊泡中miRNAs的表达差异。通过超速离心法从SW480、SW620、SW1116、HCT116结直肠癌细胞系及正常CCD841CoN细胞系培养上清中分离细胞外囊泡，并依据MISEV2023指南进行表征。采用小RNA测序技术分析miRNAs表达谱，并通过定量PCR验证。分析发现22种在结直肠癌细胞系中高表达且能有效转运至肿瘤囊泡的共同miRNAs，包括miR-9-5p、miR-182-5p、miR-196b-5p、miR-200b-5p、miR-200c-3p、miR-425-5p和miR-429，这些miRNAs与结直肠癌细胞的发育、增殖、侵袭和迁移以及囊泡成熟和转运相关通路密切相关。与此同时，正常细胞表达的miRNAs（如miR-369和miR-143）在促炎反应和肿瘤抑制中发挥作用。对结直肠癌患者血浆来源囊泡及肿瘤样本中特定miRNAs的分析显示，患者样本与结直肠癌细胞系的miRNA表达谱具有相似性。此外，在患者血浆分离的多个囊泡样本中检测到miR-182-5p、miR-196-5p、miR-425-5p和miR-429的表达。我们的研究结果表明，miR-182-5p、miR-196b-5p和miR-429在结直肠癌患者与健康供体的细胞外囊泡中存在差异表达，这可能具有临床意义。
肿瘤(癌症)患者之家