Hypoxia can modulate the immune system by affecting the function and activity of immune cells, potentially leading to altered immune responses. This study investigated the generation of leukemia-derived dendritic cells (DCleu)from leukemic blasts and their impact on immune cell activation under hypoxic (5–10% O2) compared to normoxic (21% O2) conditions using various immunomodulatory Kits. The results revealed that DC/DCleu-generation was similar under hypoxic and normoxic conditions, with no significant differences observed in frequencies of generated DC/DCleu. Furthermore, the study showed that the activation of immune cells and their anti-leukemic activity improved when T cell-enriched immunoreactive cells were co-cultured with DC/DCleuwhich were generated with Kit I and M compared to the control after mixed lymphocyte cultures. The anti-leukemic activity was improved under hypoxic compared to normoxic conditions after MLCWB-DC Kit M. These findings suggest that DC/DCleu-cultures of leukemic whole blood with Kits under hypoxic conditions yield comparable frequencies of DC/DCleuand can even increase the anti-leukemic activity compared to normoxic conditions. Overall, this research highlights the potential of utilizing DC/DCleu(potentially induced in vivo with Kits) as a promising approach to enhance immune response in patients with acute myeloid leukemia.
缺氧可通过影响免疫细胞的功能与活性来调节免疫系统，可能导致免疫应答发生改变。本研究采用多种免疫调节试剂盒，在缺氧（5-10% O2）与常氧（21% O2）条件下，探讨了白血病原始细胞来源的树突状细胞（DCleu）的生成及其对免疫细胞活化的影响。结果显示，缺氧与常氧条件下DC/DCleu的生成情况相似，生成频率未见显著差异。进一步研究发现，与对照组相比，使用试剂盒I和M生成的DC/DCleu与富集T细胞的免疫反应细胞共培养后，经混合淋巴细胞培养可增强免疫细胞活化及其抗白血病活性。特别是在使用MLCWB-DC试剂盒M后，缺氧条件下的抗白血病活性较常氧条件有所提升。这些发现表明，在缺氧条件下使用试剂盒培养白血病全血获得的DC/DCleu，其生成频率与常氧条件相当，甚至能增强抗白血病活性。总体而言，本研究揭示了利用DC/DCleu（可能通过试剂盒在体内诱导）作为增强急性髓系白血病患者免疫应答的潜在策略。
肿瘤(癌症)患者之家