文章：

LMO2 mRNA显色原位杂交表达与LMO2蛋白及基因表达相关，并揭示其在非特指型弥漫性大B细胞淋巴瘤中的生存影响

ChromogenicLMO2mRNA ISH Expression Correlates with LMO2 Protein and Gene Expression and Captures Their Survival Impact in Diffuse Large B-Cell Lymphoma, NOS

原文发布日期：28 June 2024

DOI: 10.3390/cancers16132378

类型: Article

开放获取: 是

英文摘要：

Background:LMO2is a relevant gene involved in B-cell ontogeny and a survival predictor of aggressive large B-cell lymphomas (aLBCL). Most studies assessingLMO2mRNA expression have relied on microarray platforms or qRT-PCR methods, overlooking tissue morphology. In this study, we evaluateLMO2RNA expression by chromogenic in situ hybridization (CISH) in normal tissue and in a series of 82 aLBCL. Methods:LMO2CISH was performed in formalin-fixed paraffin-embedded tissues, scored by three different methods, and correlated with a transcriptome panel. Results: We obtained statistically significant results correlating the methods of evaluation with LMO2 protein expression and gene expression results. Normal tonsil tissue showed high levels ofLMO2, particularly within the light zone of the germinal center. Conversely, in aLBCL, a notable reduction inLMO2expression was noted, remarkably in cases carryingMYCrearrangements. Furthermore, significant results were obtained through overall survival and Cox regression survival analysis, incorporating International Prognostic Index data alongsideLMO2expression levels. Conclusions: We show a reliable method to identifyLMO2mRNA expression by CISH, effectively capturing many of the reported biologic features of LMO2.

摘要翻译： 

背景：LMO2是参与B细胞个体发育的关键基因，也是侵袭性大B细胞淋巴瘤（aLBCL）的生存预测因子。目前大多数评估LMO2 mRNA表达的研究依赖于微阵列平台或qRT-PCR方法，忽略了组织形态学特征。本研究通过显色原位杂交（CISH）技术评估正常组织及82例aLBCL样本中LMO2 RNA的表达情况。方法：在福尔马林固定石蜡包埋组织中进行LMO2 CISH检测，采用三种不同评分方法，并与转录组分析结果进行关联分析。结果：评估方法与LMO2蛋白表达及基因表达结果之间存在统计学显著相关性。正常扁桃体组织显示高水平的LMO2表达，尤其在生发中心明区。相反，在aLBCL中观察到LMO2表达显著降低，特别是在携带MYC重排的病例中。此外，通过结合国际预后指数数据与LMO2表达水平进行总生存期分析和Cox回归生存分析，获得了具有统计学意义的结果。结论：本研究证实了通过CISH技术检测LMO2 mRNA表达的可靠方法，该方法能有效捕捉LMO2已知的生物学特征。

原文链接：

ChromogenicLMO2mRNA ISH Expression Correlates with LMO2 Protein and Gene Expression and Captures Their Survival Impact in Diffuse Large B-Cell Lymphoma, NOS