文章：

牛源性肉奶因子样序列在肾细胞癌组织中频繁检出

Bovine Meat and Milk Factor-like Sequences Are Frequently Detected in Renal Cell Carcinoma Tissues

原文发布日期：29 April 2024

DOI: 10.3390/cancers16091746

类型: Article

开放获取: 是

英文摘要：

Previous studies have indicated a potential role of diet in the pathogenesis of renal cell carcinoma (RCC). Recently, circular bovine meat and milk factor (BMMF) DNAs have been identified in peritumoral tissues of human colon and breast cancers. Here, we investigated the prevalence of the DNA of these novel human pathogenic infectious agents in RCC and adjacent peritumoral renal tissues. DNA was extracted from formalin-fixed and paraffin-embedded (FFPE) RCC and peritumoral kidney tissues, including a test (n = 11) and a validation (n = 152) collection. BMMF1 and BMMF2 consensus primers were designed to screen for the presence of BMMF1- and BMMF2-like DNA. In addition, BMMF-specific PCR was performed on selected cases to test for the presence of additional regions of BMMF1 and BMMF2 genomes. A reference collection of hepatocellular carcinomas (HCCs; n = 60) and adjacent peritumoral liver tissues (n = 50) was also included. Our results demonstrated that BMMF1 and BMMF2 DNAs are frequently found in human RCC tissues and are particularly more prevalent in peritumoral kidney tissues. Of note, BMMF1 and BMMF2 genotype heterogeneity was higher in peritumoral kidney tissues compared to RCC tissues. This is the first study to directly test human FFPE tissues for BMMF1- and BMMF2-like DNA using consensus PCR and demonstrate BMMF DNA in neoplastic and peritumoral kidney tissues. The findings are in line with the recently proposed indirect etiopathogenetic role of BMMFs in, e.g., colorectal carcinogenesis. Follow-up studies are needed to explore the potential role of BMMFs in the etiopathogenesis of RCC.

摘要翻译： 

既往研究表明饮食在肾细胞癌（RCC）的发病机制中可能具有潜在作用。近期研究在人类结肠癌和乳腺癌的瘤周组织中鉴定出环状牛源肉奶因子（BMMF）DNA。本研究旨在探究这些新型人类致病性感染因子DNA在RCC及邻近瘤周肾组织中的分布情况。研究从福尔马林固定石蜡包埋（FFPE）的RCC及瘤周肾组织中提取DNA，包括测试集（n=11）和验证集（n=152）。通过设计BMMF1和BMMF2共有引物，筛查BMMF1样和BMMF2样DNA的存在。此外，对部分病例进行BMMF特异性PCR以检测BMMF1和BMMF2基因组其他区域。研究同时纳入了肝细胞癌（HCC；n=60）及邻近瘤周肝组织（n=50）作为参照组。结果显示BMMF1和BMMF2 DNA在人类RCC组织中普遍存在，且在瘤周肾组织中的检出率更高。值得注意的是，与RCC组织相比，瘤周肾组织中BMMF1和BMMF2基因型异质性更高。本研究首次采用共有引物PCR技术直接检测人类FFPE组织中BMMF1样和BMMF2样DNA，并证实了BMMF DNA在肾脏肿瘤及瘤周组织中的存在。该发现与近期提出的BMMF在结直肠癌发生中的间接病因学作用相吻合。后续研究需进一步探索BMMF在RCC病因发病机制中的潜在作用。

原文链接：

Bovine Meat and Milk Factor-like Sequences Are Frequently Detected in Renal Cell Carcinoma Tissues