Background: The advancement of multidisciplinary treatment has increased the need to develop tests to monitor tumor burden during treatment. We herein analyzed urinary microRNAs within extracellular vesicles from patients with esophageal squamous cell carcinoma (ESCC) and normal individuals using a microarray.Methods:Patients with advanced ESCC who underwent esophagectomy (A), endoscopic submucosal resection (ESD) (B), and healthy donors (C) were included. Based on microRNA expression among the groups (Analysis 1), microRNAs with significant differences between groups A and C were selected (Analysis 2). Of these candidates, microRNAs in which the change between A and C was consistent with the change between B and C were selected for downstream analysis (Analysis 3). Finally, microRNA expression was validated in patients with recurrence from A (exploratory analysis).Results:For analysis 1, 205 microRNAs were selected. For Analyses 2 and 3, the changes in 18 microRNAs were consistent with changes in tumor burden as determined by clinical imaging and pathological findings. The AUC for the detection of ESCC using 18 microRNAs was 0.72. In exploratory analysis, three of eighteen microRNAs exhibited a concordant trend with recurrence.Conclusions:The current study identified the urinary microRNAs which were significantly expressed in ESCC patients. Validation study is warranted to evaluate whether these microRNAs could reflect tumor burden during multidisciplinary treatment for ESCC.
背景：随着多学科综合治疗的进展，开发用于监测治疗期间肿瘤负荷的检测方法的需求日益增加。本研究通过微阵列技术分析了食管鳞状细胞癌（ESCC）患者与正常个体尿液细胞外囊泡中的微小RNA（microRNA）。方法：研究纳入接受食管切除术的晚期ESCC患者（A组）、接受内镜黏膜下剥离术（ESD）的患者（B组）以及健康供者（C组）。基于各组间的microRNA表达差异（分析1），筛选出A组与C组间存在显著差异的microRNA（分析2）。从这些候选分子中，进一步选取在A组与C组间的变化趋势与B组和C组间变化一致的microRNA进行下游分析（分析3）。最后，在A组复发患者中对microRNA表达进行了验证性探索分析。结果：分析1共筛选出205个microRNA。在分析2和3中，有18个microRNA的变化与临床影像学和病理学评估的肿瘤负荷变化一致。使用这18个microRNA检测ESCC的曲线下面积（AUC）为0.72。探索性分析显示，18个microRNA中有3个的表达变化与肿瘤复发趋势相符。结论：本研究鉴定出在ESCC患者尿液中显著表达的microRNA。需进一步开展验证性研究，以评估这些microRNA是否能够反映ESCC多学科综合治疗过程中的肿瘤负荷变化。
Identifying the Trends of Urinary microRNAs within Extracellular Vesicles for Esophageal Cancer
肿瘤(癌症)患者之家