Background: This study aimed to investigate the effects of tetrahydrolipstatin (orlistat) on heterotopic glioblastoma in mice by applying MRI and correlating the results with histopathology and immunochemistry. Methods: Human glioblastoma cells were injected subcutaneously into the groins of immunodeficient mice. After tumor growth of >150 mm3, the animals were assigned into a treatment group (n = 6), which received daily intraperitoneal injections of orlistat, and a control group (n = 7). MRI was performed at the time of randomization and before euthanizing the animals. Tumor volumes were calculated, and signal intensities were analyzed. The internal tumor structure was evaluated visually and with texture analysis. Western blotting and protein expression analysis were performed. Results: At histology, all tumors showed high mitotic and proliferative activity (Ki67 ≥ 10%). Reduced fatty acid synthetase expression was measured in the orlistat group (p< 0.05). Based on the results of morphologic MRI-based analysis, tumor growth remained concentric in the control group and changed to eccentric in the treatment group (p< 0.05). The largest area under the receiver operating curve of the predictors derived from the texture analysis of T2w images was for wavelet transform parameters WavEnHL_s3 and WavEnLH_s4 at 0.96 and 1.00, respectively. Conclusions: Orlistat showed effects on heterotopically implanted glioblastoma multiforme in MRI studies of mice based on morphologic and texture analysis.
背景：本研究旨在通过应用磁共振成像（MRI）技术，并关联组织病理学与免疫化学结果，探讨四氢脂抑素（奥利司他）对小鼠异位胶质母细胞瘤的影响。方法：将人胶质母细胞瘤细胞皮下注射至免疫缺陷小鼠腹股沟区。待肿瘤体积增长至大于150 mm³后，将动物分为治疗组（n=6）和对照组（n=7）。治疗组每日接受腹腔注射奥利司他。在随机分组时及安乐死前分别进行MRI扫描，计算肿瘤体积并分析信号强度。通过视觉评估和纹理分析评估肿瘤内部结构，同时进行蛋白质印迹和蛋白表达分析。结果：组织学检测显示所有肿瘤均呈现较高的有丝分裂和增殖活性（Ki67 ≥ 10%）。奥利司他治疗组的脂肪酸合成酶表达显著降低（p<0.05）。基于形态学MRI分析结果显示，对照组肿瘤保持同心圆式生长，而治疗组则转变为偏心性生长（p<0.05）。在T2加权图像纹理分析预测指标中，小波变换参数WavEnHL_s3和WavEnLH_s4的受试者工作特征曲线下面积最大，分别达到0.96和1.00。结论：通过形态学和纹理分析的小鼠MRI研究表明，奥利司他对异位植入的多形性胶质母细胞瘤具有显著影响。
肿瘤(癌症)患者之家