The regulation of apoptosis is the primary goal of ablation therapy. Irreversible electroporation (IRE) is a promising non-thermal tissue ablation-based therapy that induces apoptosis by manipulating electrical conditions. This study aimed to investigate IRE-induced gastric tissue apoptosis in response to changes in the electric field intensity, followed by the repair process. Among the 52 rats used in this study, 24 were used to explore apoptosis, and 28 were used to study regeneration. The apoptosis-to-necrosis ratio of the electrical field strength was evaluated using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling and caspase-3 immunohistochemistry. The size of IRE-induced ulcers in the gastric tissue continuously increased with increasing electrical intensity (r2= 0.830,p< 0.001). The level of apoptosis gradually decreased after peaking at 200 V (1000 V/cm). The size of the 400 V-ablated ulcers continued to decrease, and they were not visible by day 14. The proliferation and migration of epithelial cells with fibroblasts were observed on day 3 and augmented on day 7 post-ablation. This investigation demonstrated the biphasic activation of apoptosis with respect to the electrical field strength. Visually and histologically, IRE-induced gastric ulcers demonstrated complete tissue regeneration after two weeks.
消融治疗的主要目标是调控细胞凋亡。不可逆电穿孔是一种基于非热组织消融的有前景的治疗方法，通过调控电学条件诱导细胞凋亡。本研究旨在探讨不可逆电穿孔诱导的胃组织凋亡对电场强度变化的响应及其后续修复过程。实验共使用52只大鼠，其中24只用于凋亡研究，28只用于再生研究。通过末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记及半胱天冬酶-3免疫组化技术，评估不同电场强度下的凋亡-坏死比例。随着电场强度增加，不可逆电穿孔诱导的胃组织溃疡面积持续增大（r²=0.830，p<0.001）。细胞凋亡水平在200V（1000V/cm）时达到峰值后逐渐下降。400V消融形成的溃疡面积持续缩小，至第14天已不可见。消融后第3天观察到成纤维细胞伴随上皮细胞增殖迁移，第7天该现象显著增强。本研究证实细胞凋亡随电场强度呈现双相激活特征。形态学与组织学观察显示，不可逆电穿孔诱导的胃溃疡在两周后实现了完全的组织再生。
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