文章：

在小鼠鳞状细胞癌模型中，通过腺病毒介导的B7-1基因转移与抗程序性细胞死亡-1抗体联合诱导免疫抗肿瘤效应

Induction of Immunological Antitumor Effects by the Combination of Adenovirus-Mediated Gene Transfer of B7-1 and Anti-Programmed Cell Death-1 Antibody in a Murine Squamous Cell Carcinoma Model

原文发布日期：30 March 2024

DOI: 10.3390/cancers16071359

类型: Article

开放获取: 是

英文摘要：

Background: The goal of this study was to evaluate the antitumor immune effects of B7-1 gene expression in addition to immune checkpoint inhibitor against squamous cell carcinoma. Methods: A murine SCC cell line, KLN205, was infected with adenoviral vector carrying B7-1 (AdB7). Infected cells were injected subcutaneously in the flanks of DBA/2 mice. Three weeks after implantation, anti-mouse PD-1 antibody (antiPD1) was intraperitonially administrated twice a week for a total of six times. Results: CD80 was significantly overexpressed in the AdB7-infected tumors. IFN-gamma in the T cells in the spleen was significantly increased and tumor size was significantly reduced in the mice treated with both AdB7 and antiPD1. Targeted tumors treated with both AdB7 and antiPD1 exhibited significantly increased cell densities of total immune cells as well as Ki-67+ CD8+ T cells and decreased regulatory T cells. Conclusions: These results suggest that the B7-1 gene transfer may enhance the antitumor effect of anti-PD1 antibody against SCC.

摘要翻译： 

背景：本研究旨在评估B7-1基因表达联合免疫检查点抑制剂对鳞状细胞癌的抗肿瘤免疫效应。方法：采用携带B7-1基因的腺病毒载体（AdB7）感染小鼠鳞癌细胞系KLN205，将感染细胞皮下注射至DBA/2小鼠侧腹。移植三周后，腹腔注射抗小鼠PD-1抗体（antiPD1），每周两次，共计六次。结果：AdB7感染肿瘤中CD80表达显著上调。联合应用AdB7与antiPD1治疗的小鼠脾脏T细胞中IFN-γ水平显著升高，肿瘤体积明显缩小。联合治疗组的靶向肿瘤组织中，总免疫细胞密度及Ki-67+ CD8+ T细胞密度显著增加，调节性T细胞数量减少。结论：B7-1基因转导可能增强抗PD-1抗体对鳞状细胞癌的抗肿瘤效应。

原文链接：

Induction of Immunological Antitumor Effects by the Combination of Adenovirus-Mediated Gene Transfer of B7-1 and Anti-Programmed Cell Death-1 Antibody in a Murine Squamous Cell Carcinoma Model