Non-melanoma skin cancer (NMSC) is the most prevalent cancer in humans, with a high global incidence. We present a prospective clinical feasibility study on the use of intraoperative flow cytometry (iFC) for the instant diagnosis of NMSC and its complete surgical clearance. Flow cytometry, a laser-based technique, quantifies cell features, which has applications in cancer research. This study aim is to explore the potential applicability of iFC in detecting and characterizing NMSC and its surgical margins. In total, 30 patients who underwent diagnosis for NMSC were recruited. The method demonstrated high sensitivity (95.2%) and specificity (87.1%), with an accuracy of 91.1%, as confirmed with a receiver operating characteristic curve analysis. The results also indicated that most tumors were diploid, with two cases being hypoploid. The average G0/G1 fractions for normal and tumor tissue samples were 96.03 ± 0.30% and 88.03 ± 1.29%, respectively, with the tumor index escalating from 3.89 ± 0.30% to 11.95 ± 1.29% in cancerous cells. These findings underscore iFC’s capability for precise intraoperative NMSC characterization and margin evaluation, promising enhanced complete tumor excision rates. Given the technique’s successful application in various other malignancies, its implementation in NMSC diagnosis and treatment holds significant promise and warrants further research in clinical trials.
非黑色素瘤皮肤癌（NMSC）是人类最常见的恶性肿瘤，全球发病率居高不下。本研究旨在探讨术中流式细胞术（iFC）在NMSC即时诊断及手术彻底切除中的应用前景，是一项前瞻性临床可行性研究。流式细胞术作为一种基于激光的技术，可量化细胞特征，在癌症研究中具有重要应用价值。本研究共纳入30例接受NMSC诊断的患者。受试者工作特征曲线分析证实，该方法具有高灵敏度（95.2%）和高特异度（87.1%），准确率达91.1%。结果显示大多数肿瘤为二倍体，其中两例为亚二倍体。正常组织与肿瘤组织的平均G0/G1期细胞比例分别为96.03±0.30%和88.03±1.29%，而肿瘤指数在癌细胞中从3.89±0.30%显著上升至11.95±1.29%。这些发现充分证明iFC能够实现术中NMSC的精准表征和切缘评估，有望提高肿瘤完全切除率。鉴于该技术已在多种其他恶性肿瘤中成功应用，其在NMSC诊疗领域的实施前景广阔，值得在临床试验中开展进一步深入研究。
肿瘤(癌症)患者之家