Head and neck mucosal melanoma is one of the most common types of melanoma in China, but the prognosis is worse than other types, and there is no effective treatment plan to improve patient survival. This study analyzes the efficacy of hypofractionation radiotherapy combined with PD-1 inhibitor in the treatment of head and neck mucosal melanoma, as well as its impact on the tumor immune microenvironment. NPSG mice were used to construct a humanized bilateral lesion tumor model of the humanized immune system. The models were divided into an RT (8 Gy)+anti PD-1 group, an RT (2 GyX4)+anti PD-1 group, an Anti PD-1 group, an RT (8 Gy) group, and a blank group. Differences in efficacy and immune cells in blood, lymph nodes, and tumor tissues were compared between different treatment groups. The treatment effect of RT (8 Gy)+anti PD-1 was better than the other groups with a tumor growth inhibition value (TGI) over 60%. Significant recruitment and activation of CD8+T cells were found in the blood, lymph nodes, and tumor tissues and significantly inhibited the level of PD-1+CD8+T cells in the group of RT (8 Gy)+anti PD-1. This study confirmed the efficacy of hypofractionation radiotherapy combined with PD-1 inhibitors, which can inhibit tumor growth and produce distant effects. The appearance of a distant effect is related to the enhancement in the number and activity of CD8+T cells in the local tumor and peripheral blood and lymph nodes. This study confirms the therapeutic and immune regulatory effect of hypofractionation radiotherapy combined with PD-1 inhibitors.
头颈部黏膜黑色素瘤是我国最常见的黑色素瘤类型之一，但预后较其他类型更差，目前尚无有效治疗方案可提高患者生存率。本研究分析了大分割放疗联合PD-1抑制剂治疗头颈部黏膜黑色素瘤的疗效及其对肿瘤免疫微环境的影响。采用NPSG小鼠构建人源化免疫系统的人源化双侧病灶肿瘤模型，将模型分为RT（8 Gy）+抗PD-1组、RT（2 GyX4）+抗PD-1组、抗PD-1组、RT（8 Gy）组和空白组，比较不同治疗组间疗效及血液、淋巴结和肿瘤组织中免疫细胞的差异。RT（8 Gy）+抗PD-1组的治疗效果优于其他组，肿瘤生长抑制值（TGI）超过60%。在RT（8 Gy）+抗PD-1组中，血液、淋巴结和肿瘤组织中均发现CD8+T细胞显著募集和活化，并显著抑制了PD-1+CD8+T细胞水平。本研究证实了大分割放疗联合PD-1抑制剂的疗效，可抑制肿瘤生长并产生远端效应。远端效应的出现与局部肿瘤及外周血和淋巴结中CD8+T细胞数量和活性的增强有关。本研究证实了大分割放疗联合PD-1抑制剂的治疗和免疫调节作用。
肿瘤(癌症)患者之家