Background: Tumor budding (TB) is a histomorphological characteristic of the tumor invasion front and it has an impact on the tumor outcome prediction for head and neck squamous cell carcinoma (HNSCC) aetiopathology. Patients and methods: The average TB score (TB rel) of all tumor-positive marginal sections (n = 443) in the primary tumor was analyzed in the FFPE-fixed tumor slices of 66 patients with HNSCC, and they were compared with cryo-fixed sections. Results: TB rel correlates with tumor aggressiveness (i.e., lymph node metastasis quantity, lymph node ratio, extra capsular growth, Pn1, pV1, grading). The TB scores often vary between the different tumor margins of FFPE sections in the same patient, and in many cases, they differ depending on the fixation method. Conclusion: Our data show that a randomly selected marginal cut cannot reliably mirror the TB score, and thus, they cannot predict the prognostic outcome. However, TB rel could be a tool that compensates for differences in TB score analysis. TB score determination in cryo sections seems to be inaccurate compared with TB determination in FFPE.
背景：肿瘤出芽（TB）是肿瘤侵袭前沿的一种组织形态学特征，对头颈部鳞状细胞癌（HNSCC）病因病理学的肿瘤预后预测具有重要影响。患者与方法：在66例HNSCC患者的福尔马林固定石蜡包埋（FFPE）肿瘤切片中，分析了原发肿瘤所有肿瘤阳性切缘切片（n = 443）的平均TB评分（TB rel），并与冷冻固定切片进行了比较。结果：TB rel与肿瘤侵袭性（即淋巴结转移数量、淋巴结比率、包膜外生长、Pn1、pV1、分级）相关。同一患者FFPE切片的不同肿瘤切缘之间的TB评分常常存在差异，并且在许多情况下，这些差异取决于固定方法。结论：我们的数据表明，随机选择的切缘切片无法可靠地反映TB评分，因此无法预测预后结果。然而，TB rel可能是一种能够弥补TB评分分析差异的工具。与FFPE切片中的TB测定相比，冷冻切片中的TB评分测定似乎不够准确。
New Tumor Budding Evaluation in Head and Neck Squamous Cell Carcinomas
肿瘤(癌症)患者之家