文章：

常规小动物辐照器中的体内微束放射治疗

In Vivo Microbeam Radiation Therapy at a Conventional Small Animal Irradiator

原文发布日期：30 January 2024

DOI: 10.3390/cancers16030581

类型: Article

开放获取: 是

英文摘要：

Microbeam radiation therapy (MRT) is a still pre-clinical form of spatially fractionated radiotherapy, which uses an array of micrometer-wide, planar beams of X-ray radiation. The dose modulation in MRT has proven effective in the treatment of tumors while being well tolerated by normal tissue. Research on understanding the underlying biological mechanisms mostly requires large third-generation synchrotrons. In this study, we aimed to develop a preclinical treatment environment that would allow MRT independent of synchrotrons. We built a compact microbeam setup for pre-clinical experiments within a small animal irradiator and present in vivo MRT application, including treatment planning, dosimetry, and animal positioning. The brain of an immobilized mouse was treated with MRT, excised, and immunohistochemically stained againstγH2AX for DNA double-strand breaks. We developed a comprehensive treatment planning system by adjusting an existing dose calculation algorithm to our setup and attaching it to the open-source software 3D-Slicer. Predicted doses in treatment planning agreed within 10% with film dosimetry readings. We demonstrated the feasibility of MRT exposures in vivo at a compact source and showed that the microbeam pattern is observable in histological sections of a mouse brain. The platform developed in this study will be used for pre-clinical research of MRT.

摘要翻译： 

微束放射治疗（MRT）是一种仍处于临床前阶段的空间分割放射治疗形式，其采用微米级宽度的平面X射线束阵列。研究证实，MRT的剂量调制在有效治疗肿瘤的同时，对正常组织具有良好的耐受性。目前理解其潜在生物学机制的研究主要依赖大型第三代同步辐射装置。本研究旨在建立一种不依赖同步辐射装置的临床前治疗环境。我们在小动物辐照仪内构建了紧凑型微束实验装置，并实现了活体MRT应用，包括治疗计划制定、剂量测定和动物定位。通过对固定小鼠脑部实施MRT照射后，取出脑组织进行针对DNA双链断裂标志物γH2AX的免疫组化染色。我们通过调整现有剂量计算算法以适应本实验装置，并将其整合至开源软件3D-Slicer中，开发出完整的治疗计划系统。治疗计划中的预测剂量与胶片剂量测定读数误差保持在10%以内。本研究证实了在紧凑型辐射源上实施活体MRT照射的可行性，并证明小鼠脑组织切片中可观察到微束辐照模式。该研究构建的平台将为MRT的临床前研究提供重要工具。

原文链接：

In Vivo Microbeam Radiation Therapy at a Conventional Small Animal Irradiator