The spleen, traditionally associated with blood filtration and immune surveillance, has recently been recognized for its role in systemic lipid metabolism and potential influence on cancer development and progression. This study investigates effects of dietary supplements, specifically conjugated linolenic acids from pomegranate seed oil and bitter melon extract, on the fatty acid (FA) composition of the spleen in the context of cancerous processes. Advanced methods, including gas chromatography–mass spectrometry and silver ion-impregnated high-performance liquid chromatography, were employed to analyze the spleen’s FA profile. Our research uncovered that dietary supplementation leads to alterations in the spleen’s FA profile, especially under the carcinogenic influence of 7,12-dimethylbenz[a]anthracene. These changes did not align with a simple protective or anti-carcinogenic pattern, as previously suggested in in vitro studies. We observed shifts in conjugated FA isomer concentrations and variations in desaturase activities, suggesting disrupted lipid metabolism in cancerous conditions. The findings underscore the spleen’s vital role in lipid metabolism within the body’s systemic health framework, highlighting the complexity of dietary supplements’ impact on FA profiles in the spleen and their potential implications in cancer progression and treatment. This study adds valuable insight into the complex interplay between diet, disease, and metabolic regulation, particularly in cancerous environments.
传统上被认为与血液过滤和免疫监视相关的脾脏，近期被发现其在全身脂质代谢中扮演重要角色，并可能影响癌症的发生与发展。本研究探讨了膳食补充剂——特别是来自石榴籽油的共轭亚麻酸和苦瓜提取物——在癌变过程中对脾脏脂肪酸组成的影响。研究采用气相色谱-质谱联用技术和银离子浸渍高效液相色谱等先进方法，分析了脾脏的脂肪酸谱。研究发现，膳食补充会导致脾脏脂肪酸谱的改变，尤其是在7,12-二甲基苯并[a]蒽的致癌影响下。这些变化并未呈现先前体外研究中提出的简单保护或抗癌模式。我们观察到共轭脂肪酸异构体浓度的变化以及去饱和酶活性的波动，表明在癌变条件下脂质代谢受到干扰。这些发现强调了脾脏在全身健康框架内脂质代谢中的关键作用，揭示了膳食补充剂对脾脏脂肪酸谱影响的复杂性及其在癌症进展和治疗中的潜在意义。本研究为饮食、疾病与代谢调控之间复杂的相互作用，特别是在癌变环境中的关系，提供了有价值的见解。
肿瘤(癌症)患者之家