The goal of this paper was the evaluation of the changes in the expression profile of irisin, ghrelin, and titin in the carcinoma tissue and in the blood of patients with head and neck squamous cell carcinoma (HNSCC), including determining the profile of their expression in relation to patient nutrition. The study included 56 patients with diagnosed squamous cell carcinoma of HNSCC in the T3 and T4 stages of the disease. Healthy control tissue specimens were collected from an area 10 mm outside the histologically negative margin. In turn, the blood and serum from the control group came from healthy volunteers treated for non-oncologic reasons (n = 70). The molecular analysis allowed us to determine the profile of irisin, ghrelin, and titin methylation, evaluate their expression on the level of mRNA (quantitative Reverse Transcription Polymerase Chain Reaction; qRT-PCR) and protein (Enzyme-Linked Immunosorbent Assay Reaction; ELISA) in the carcinoma tissue and the margin of healthy tissue, as well as in serum of patients in the study and control groups. At the start of our observations, a Body Mass Index (BMI) < 18.5 was noted in 42 of the patients, while six months after the treatment a BMI < 18.5 was noted in 29 patients. We also noted a decrease in the expression of irisin, ghrelin, and titin both on the level of mRNA and protein, as well as a potential regulation of their expression via DNA methylation. There is no convincing evidence that the proteins assayed in the present work are specific with regard to HNSSC.
本研究旨在评估头颈部鳞状细胞癌（HNSCC）患者癌组织及血液中鸢尾素、胃饥饿素和肌联蛋白表达谱的变化，并分析其表达谱与患者营养状况的关系。研究纳入了56例确诊为T3和T4期HNSCC的患者。健康对照组织标本取自组织学阴性边缘外10mm区域。对照组血液和血清则来自因非肿瘤原因接受治疗的健康志愿者（n=70）。通过分子分析，我们确定了鸢尾素、胃饥饿素和肌联蛋白的甲基化谱，评估了它们在癌组织、健康组织边缘以及研究组和对照组患者血清中mRNA（定量逆转录聚合酶链反应；qRT-PCR）和蛋白质（酶联免疫吸附反应；ELISA）水平的表达。观察初期，42例患者体重指数（BMI）<18.5，治疗六个月后，29例患者BMI<18.5。我们还观察到鸢尾素、胃饥饿素和肌联蛋白在mRNA和蛋白质水平的表达均有所下降，且其表达可能受DNA甲基化调控。目前尚无确凿证据表明本研究检测的蛋白质对HNSCC具有特异性。
肿瘤(癌症)患者之家