Traditional microbiological methodology is valuable and essential for microbiota composition description and microbe role assignations at different anatomical sites, including cervical and vaginal tissues; that, combined with molecular biology strategies and modern identification approaches, could give a better perspective of the microbiome under different circumstances. This pilot work aimed to describe the differences in microbiota composition in non-cancer women and women with cervical cancer through a culturomics approach combining culture techniques with Vitek mass spectrometry and 16S rDNA sequencing. To determine the possible differences, diverse statistical, diversity, and multivariate analyses were applied; the results indicated a different microbiota composition between non-cancer women and cervical cancer patients. The Firmicutes phylum dominated the non-cancer (NC) group, whereas the cervical cancer (CC) group was characterized by the predominance of Firmicutes and Proteobacteria phyla; there was a depletion of lactic acid bacteria, an increase in the diversity of anaerobes, and opportunistic and non-typical human microbiota isolates were present. In this context, we hypothesize and propose a model in which microbial composition and dynamics may be essential for maintaining the balance in the cervical microenvironment or can be pro-oncogenesis microenvironmental mediators in a process calledYing-Yangor have a protagonist/antagonist microbiota role.
传统微生物学方法对于描述不同解剖部位（包括宫颈和阴道组织）的微生物群组成及确定微生物作用具有重要价值，结合分子生物学策略与现代鉴定技术，能够更全面地揭示不同状态下微生物组的特征。本项探索性研究旨在通过培养组学方法，结合培养技术、Vitek质谱鉴定与16S rDNA测序，描述非癌症女性与宫颈癌患者微生物群组成的差异。研究采用多种统计学分析、多样性分析与多变量分析方法以确定潜在差异，结果显示两组人群的微生物群组成存在显著不同。非癌症组以厚壁菌门为主导，而宫颈癌组则呈现厚壁菌门与变形菌门共优势的特征；同时观察到乳酸菌数量减少、厌氧菌多样性增加，并存在机会性致病菌与非典型人体微生物分离株。基于此，我们提出假说并构建模型，认为微生物组成及其动态变化可能对维持宫颈微环境平衡具有关键作用，或在称为"阴阳"的过程中成为促癌发生的微环境介质，从而在微生物群中扮演主导/拮抗的角色。
Cultivable Microbiome Approach Applied to Cervical Cancer Exploration
肿瘤(癌症)患者之家