文章：

激肽受体及激肽相关基因在星形细胞脑肿瘤中的表达

Kinin Receptors and Kinin-Related Gene Expression in Astrocytic Brain Tumors

原文发布日期：5 January 2024

DOI: 10.3390/cancers16020241

类型: Article

开放获取: 是

英文摘要：

Kinins are a set of peptides present in tissues that are involved in the inflammatory response and cancer progression. However, studies showing the expression of kinin receptors in human glioma samples are still incomplete and contradictory. The aim of the present study was to ascertain the expression ofBDKRB1andBDKRB2genes, as well as the level of B1R and B2R proteins in human gliomas, depending on the degree of malignancy. Additionally, representative kinin-dependent genes with altered expression were indicated. The expression profile of kinin-dependent genes was determined using oligonucleotide microarray technique. In addition, RT-qPCR was used to assess the expression level of selected differentiating genes. The location of kinin receptors in brain gliomas was assessed using immunohistochemical methods. The oligonucleotide microarray method was used to identify 12 mRNA IDs of kinin-related genes whose expression was upregulated or downregulated in gliomas of different grades. In immunohistochemically stained samples, the concentrations of BR1 and BR2 proteins, measured by optical density, were statistically significantly higher in grade G3 vs. G2 and G4 vs. G3. Increased expression of kinin receptorsBDKRB1andBDKRB2in brain gliomas, depending on the degree of malignancy, suggests the involvement of kinins and their receptors in the disease’s pathogenesis. Quantitative assessment of mRNABDKRB1,PRKAR1A,MAP2K, andEGFRin patients with brain tumors may hold diagnostic and therapeutic significance.

摘要翻译： 

激肽是一组存在于组织中的肽类，参与炎症反应和癌症进展。然而，关于激肽受体在人类胶质瘤样本中表达的研究仍不完整且存在矛盾。本研究旨在根据恶性程度，确定人类胶质瘤中BDKRB1和BDKRB2基因的表达情况，以及B1R和B2R蛋白的水平。此外，还指出了具有表达变化的代表性激肽依赖性基因。激肽依赖性基因的表达谱通过寡核苷酸微阵列技术确定。同时，采用RT-qPCR评估了选定分化基因的表达水平。通过免疫组织化学方法评估了激肽受体在脑胶质瘤中的定位。利用寡核苷酸微阵列方法，鉴定出12个与激肽相关的mRNA ID，这些基因在不同级别的胶质瘤中表达上调或下调。在免疫组织化学染色的样本中，通过光密度测量的BR1和BR2蛋白浓度在G3级与G2级、G4级与G3级之间具有统计学显著差异。脑胶质瘤中激肽受体BDKRB1和BDKRB2的表达增加与恶性程度相关，表明激肽及其受体参与了该疾病的发病机制。对脑肿瘤患者中mRNA BDKRB1、PRKAR1A、MAP2K和EGFR的定量评估可能具有诊断和治疗意义。

原文链接：

Kinin Receptors and Kinin-Related Gene Expression in Astrocytic Brain Tumors