Understanding molecular networks of CRLM is an ongoing area of research. In this study, paired CRC tissue and adjacent noncancerous tissue from 15 non-metastatic CRC patients and paired CRC tissue and matched liver metastatic tissues from 15 CRLM patients along with their adjacent noncancerous tissues were evaluated. We assessed Rap1 pathway-related genes including NRAS, FGF-1, NGF, and KDR expression by qRT-PCR and their protein status by Western blot. In CRLM patients, NRAS, FGF1, and KDR mRNA and protein were expressed at higher levels in metastatic than in CRC primary tumor and adjacent noncancerous tissue (p< 0.05). In non-metastatic patients, NRAS, FGF1, KDR, and NGF gene expression did not differ between CRC primary tumor-and adjacent noncancerous tissue (p> 0.05). ROC curve analysis showed a reasonable diagnostic accuracy of NRAS, FGF1, KDR, and FGF for the discrimination of metastatic patients from non- metastatic ones on analysis of their primary tumors. The data suggest that further functional studies on Rap1-related genes’ role in CRLM are needed. In conclusion, the present data broaden our knowledge about specific molecular characteristics of CRLM. An increased understanding of the molecular features of metastasis has the potential to create more successful treatment, or prevention, of metastasis, especially in multimodal primary tumor treatment.
理解结直肠癌肝转移（CRLM）的分子网络是一个持续的研究领域。本研究评估了15例非转移性结直肠癌（CRC）患者的配对CRC组织与癌旁非癌组织，以及15例CRLM患者的配对CRC组织与匹配的肝转移组织及其癌旁非癌组织。我们通过qRT-PCR评估了Rap1通路相关基因（包括NRAS、FGF-1、NGF和KDR）的表达，并通过Western blot检测了其蛋白状态。在CRLM患者中，NRAS、FGF1和KDR的mRNA和蛋白在转移灶中的表达水平均高于CRC原发肿瘤和癌旁非癌组织（p < 0.05）。在非转移性患者中，NRAS、FGF1、KDR和NGF的基因表达在CRC原发肿瘤与癌旁非癌组织之间无显著差异（p > 0.05）。ROC曲线分析显示，通过对原发肿瘤的分析，NRAS、FGF1、KDR和FGF在区分转移性与非转移性患者方面具有合理的诊断准确性。数据表明，需要对Rap1相关基因在CRLM中的作用进行进一步的功能研究。总之，本研究数据拓宽了我们对CRLM特定分子特征的认识。加深对转移分子特征的理解，有望为转移提供更成功的治疗或预防策略，尤其是在多模式原发肿瘤治疗中。
肿瘤(癌症)患者之家