文章：

磷脂酶A2 IIA组与炎性肝细胞腺瘤相关

Phospholipase A2 Group IIA Is Associated with Inflammatory Hepatocellular Adenoma

原文发布日期：28 December 2023

DOI: 10.3390/cancers16010159

类型: Article

开放获取: 是

英文摘要：

Although benign hepatocellular adenomas (HCA) are very rare, recent observations have shown their occurrence in patients with diabetes mellitus. Consequently, most of these cases are treated by resection due to concerns regarding their potential progression to hepatocarcinoma (HCC). This decision is largely driven by the limited number of studies on HCC subtyping and the lack of molecular and biological insights into the carcinogenic potential of benign tumors. This study aimed to comprehensively investigate the subtype classification of HCA and to compare and analyze gene expression profiling between HCA and HCC tissues. One fresh inflammatory HCA (I-HCA), three non-B non-C HCCs, two hepatitis B virus-HCCs, and one normal liver tissue sample were subjected to single-cell RNA sequencing (scRNA-seq). Comparative analysis of scRNA-seq among different tissues showed that phospholipase A2 group IIA (PLA2G2A) mRNA was specifically expressed in I-HCA, following RNA-seq analysis in formalin-fixed paraffin-embedded tissues from other HCAs. Immunohistochemistry using the PLA2G2A antibody in these tissues indicated that the positive reaction was mainly observed in hepatocytes of I-HCAs and stromal cells surrounding the tumor tissue in HCC were also stained. According to a clinical database, PLA2G2A expression in HCC does not correlate with poor prognosis. This finding may potentially help develop a new definition for I-HCA, resulting in a significant clinical contribution, but it requires validation with other fresh HCA samples.

摘要翻译： 

尽管良性肝细胞腺瘤（HCA）非常罕见，但近期观察发现其在糖尿病患者中亦有发生。因此，由于担心其可能进展为肝细胞癌（HCC），大多数此类病例通过手术切除进行治疗。这一决策主要源于目前对HCC亚型分化的研究有限，且对良性肿瘤致癌潜能的分子与生物学机制认识不足。本研究旨在全面探究HCA的亚型分类，并对HCA与HCC组织间的基因表达谱进行比较分析。研究对一例新鲜炎症型HCA（I-HCA）、三例非乙非丙型HCC、两例乙型肝炎病毒相关HCC及一例正常肝组织样本进行了单细胞RNA测序（scRNA-seq）。不同组织间的scRNA-seq对比分析显示，磷脂酶A2 IIA组（PLA2G2A）mRNA在I-HCA中特异性表达，此结果在其他HCA的石蜡包埋组织RNA测序分析中得到验证。在这些组织中使用PLA2G2A抗体进行的免疫组化染色表明，阳性反应主要见于I-HCA的肝细胞中，同时HCC肿瘤组织周围的间质细胞也被染色。根据临床数据库分析，HCC中PLA2G2A的表达与不良预后无相关性。这一发现可能有助于为I-HCA建立新的定义标准，从而产生重要的临床价值，但尚需通过其他新鲜HCA样本进行验证。

原文链接：

Phospholipase A2 Group IIA Is Associated with Inflammatory Hepatocellular Adenoma