文章：

头颈部鳞状细胞癌中宿主-细菌相互作用的实验建模

Experimental Modeling of Host–Bacterial Interactions in Head and Neck Squamous Cell Carcinoma

原文发布日期：12 December 2023

DOI: 10.3390/cancers15245810

类型: Article

开放获取: 是

英文摘要：

The microscopic species colonizing the human body, collectively referred to as the microbiome, play a crucial role in the maintenance of tissue homeostasis, immunity, and the development of disease. There is evidence to suggest associations between alterations in the microbiome and the development of head and neck squamous cell carcinomas (HNSCC). The use of two-dimensional (2D) modeling systems has made significant strides in uncovering the role of microbes in carcinogenesis; however, direct mechanistic links remain in their infancy. Patient-derived three-dimensional (3D) HNSCC organoid and organotypic models have recently been described. Compared to 2D models, 3D organoid culture systems effectively capture the genetic and epigenetic features of parent tissue in a patient-specific manner and may offer a more nuanced understanding of the role of host–microbe responses in carcinogenesis. This review provides a topical literature review assessing the current state of the field investigating the role of the microbiome in HNSCC; including in vivo and in vitro modeling methods that may be used to characterize microbiome–epithelial interactions.

摘要翻译： 

定植于人体的微观生物群落，统称为微生物组，在维持组织稳态、免疫功能和疾病发生发展中起着至关重要的作用。现有证据表明，微生物组的改变与头颈部鳞状细胞癌（HNSCC）的发生存在关联。二维（2D）模型系统在揭示微生物于致癌过程中的作用方面已取得显著进展，但其直接机制联系的研究仍处于起步阶段。近期，基于患者来源的三维（3D）HNSCC类器官及器官型模型已被报道。相较于2D模型，3D类器官培养系统能以患者特异性的方式有效保留源组织的遗传与表观遗传特征，并可能为宿主-微生物互作在致癌过程中的作用提供更精细的解析。本文通过专题文献综述，评估当前微生物组在HNSCC中作用的研究现状，涵盖可用于表征微生物-上皮相互作用的体内外模型方法。

原文链接：

Experimental Modeling of Host–Bacterial Interactions in Head and Neck Squamous Cell Carcinoma