Salvador homolog-1 (SAV1) is a component of the Hippo pathway that regulates tissue growth and homeostasis by affecting diverse cell processes, including apoptosis, cell division, and differentiation. The aberrant expression of Hippo pathway components has been observed in various human cancers. This study aimed to examine the expression level of theSAV1gene in colorectal cancer (CRC) and its prognostic value and associations with tumor progression. We obtained matched pairs of tumor tissue and non-cancerous mucosa of the large intestine from 94 CRC patients as well as 40 colon biopsies of healthy subjects collected during screening colonoscopy. The tissue samples and CRC cell lines were quantified forSAV1mRNA levels using the quantitative polymerase chain reaction method, while SAV1 protein expression was estimated in the paired tissues of CRC patients using immunohistochemistry. The average level ofSAV1mRNA was decreased in 93.6% of the tumor tissues compared to the corresponding non-cancerous tissues and biopsies of healthy colon mucosa. A downregulated expression ofSAV1mRNA was also noted in the CRC cell lines. Although the average SAV1 immunoreactivity was increased in the CRC samples compared to the non-cancerous tissues, a decreased immunoreactivity of the SAV1 protein in the tumor specimens was associated with lymph node involvement and higher TNM disease stage and histological grade. The results of our study suggest that the impaired expression of SAV1 is involved in CRC progression.
Salvador同源物-1（SAV1）是Hippo信号通路的关键组分，该通路通过调控细胞凋亡、分裂与分化等多种细胞进程参与组织生长与稳态维持。已有研究表明Hippo通路组分在多种人类恶性肿瘤中存在异常表达。本研究旨在探讨SAV1基因在结直肠癌中的表达水平及其预后价值，并分析其与肿瘤进展的关联。我们收集了94例结直肠癌患者的癌组织与对应癌旁大肠黏膜配对样本，以及40例结肠镜筛查中获取的健康受试者结肠活检组织。采用定量聚合酶链反应技术检测组织样本及结直肠癌细胞系中SAV1 mRNA表达水平，同时通过免疫组织化学法评估结直肠癌患者配对组织中SAV1蛋白表达情况。结果显示，与对应癌旁组织及健康结肠黏膜相比，93.6%的癌组织中SAV1 mRNA平均表达水平显著降低。结直肠癌细胞系中也观察到SAV1 mRNA表达下调现象。虽然结直肠癌样本中SAV1蛋白免疫反应性平均值较非癌组织有所升高，但肿瘤标本中SAV1蛋白免疫反应性降低与淋巴结转移、更高TNM分期及组织学分级显著相关。本研究结果表明SAV1表达受损参与结直肠癌的进展过程。
肿瘤(癌症)患者之家