文章：

通过无针注射高压递送溶瘤病毒以保持治疗活性

High-Pressure Delivery of Oncolytic Viruses via Needle-Free Injection Preserves Therapeutic Activity

原文发布日期：30 November 2023

DOI: 10.3390/cancers15235655

类型: Article

开放获取: 是

英文摘要：

Intratumoural delivery of oncolytic viruses (OVs) to solid tumours is currently performed via multiple percutaneous methods of needle injections (NI). In this study, we investigated the potential use of a novel delivery approach, needle-free injection (NFI), to administer OVs to subcutaneous tumours. The stability and genetic integrity of several RNA and DNA viruses exposed to high-pressure jet injectors were first evaluated in vitro. We demonstrate that replication competence and infectivity of the viruses remained unchanged after NFI, as compared to traditional NI. Using the oncolytic Vesicular Stomatitis Virus expressing luciferase (VSVΔ51-Luc) in the syngeneic CT26 subcutaneous tumour model, we show that NFI administration not only successfully delivers infectious particles but also increases the dissemination of the virus within the tumour tissues when compared to NI. Furthermore, mice treated with VSVΔ51-Luc by NFI delivery showed similar reduction in tumour growth and survival compared to those with needle-administered virus. These results indicate that NFI represents a novel approach to administer and potentially increase the spread of OVs within accessible solid tumours, highlighting its usefulness in virotherapy.

摘要翻译： 

目前，溶瘤病毒（OVs）向实体瘤的瘤内递送主要通过多种经皮针头注射（NI）方法实现。本研究探讨了一种新型递送方式——无针注射（NFI）在皮下肿瘤中应用OVs的潜在价值。首先在体外评估了多种RNA和DNA病毒经高压喷射注射器处理后的稳定性与遗传完整性。实验证明，与传统NI相比，经NFI处理后病毒的复制能力与感染性均保持不变。在同源CT26皮下肿瘤模型中，使用表达荧光素酶的溶瘤性水疱性口炎病毒（VSVΔ51-Luc）进行实验，结果显示：与NI相比，NFI不仅能成功递送具有感染性的病毒颗粒，还能促进病毒在肿瘤组织内的扩散。此外，通过NFI递送VSVΔ51-Luc治疗的小鼠，在肿瘤生长抑制和生存期方面与针头注射病毒组表现出相似的效果。这些结果表明，NFI作为一种新型递送方式，不仅能有效投递OVs，还可能增强其在可触及实体瘤内的扩散，凸显了该方法在病毒治疗中的应用价值。

原文链接：

High-Pressure Delivery of Oncolytic Viruses via Needle-Free Injection Preserves Therapeutic Activity