文章：

墨西哥蓟叶的抗癌活性及其对TNF-α与NF-κB信号通路的影响

Cancer-Preventive Activity ofArgemone mexicanaLinn Leaves and Its Effect on TNF-α and NF-κB Signalling

原文发布日期：30 November 2023

DOI: 10.3390/cancers15235654

类型: Article

开放获取: 是

英文摘要：

Skin cancer is the 5th most common cancer in Western countries with a surge in case occurrences making it a global burden on healthcare systems. The present study aims to evaluate the cancer-preventive activity of an ethanolic extract ofArgemone mexicanaLinn leaves (AML). The DMBA/TPA method was used to induce skin cancer in mice. Experimental animals were divided into three pretreatment groups of 100 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW of AML extract, and feeding was continued during the induction process. In the fourth group, 500 mg/kg BW AML extract treatment was started along with the cancer induction. The analyses were performed on the basis of the time period of in-tumour induction incidence, haematological parameters, histopathology and augmentation of TNF-α secretion and the NF-κB (p65 subunit) signalling pathway. The AML extract resisted and delayed tumour formation for up to 8 weeks in the 500 mg/kg BW pretreated group as compared to 4 weeks in the negative control group. The tumour burden varied in a dose-dependent manner in the different groups. On the 60th day, a significantly high burden (p< 0.001) was observed in the negative control group and the 100 mg/kg BW group. The study was validated by investigating the expression of TNF-α and the p65 subunit of the NF-κB signalling pathway, which were found to be reduced significantly in a dose-dependent manner and significantly reduced (p< 0.001) in the 500 mg/kg BW group as compared to negative control group. The 500 mg/kg BW pretreated group was found to have significant results in comparison to the 500 mg/kg BW post-treatment group. The study revealed the effective cancer preventive activity ofArgemone mexicanaLinn leaves (AML) in the mouse model and paved a pathway for molecular approaches which could be explored more in future studies.

摘要翻译： 

皮肤癌在西方国家位列第五大常见癌症，其发病率激增已成为全球医疗系统的负担。本研究旨在评估墨西哥蓟叶乙醇提取物的癌症预防活性。采用DMBA/TPA法诱导小鼠皮肤癌模型，实验动物分为三个预处理组（分别灌胃100、250、500 mg/kg体重的提取物）并在诱导过程中持续给药，另设第四组在诱导开始时同步给予500 mg/kg体重提取物。通过肿瘤诱导潜伏期、血液学参数、组织病理学及TNF-α分泌与NF-κB（p65亚基）信号通路激活等指标进行分析。结果显示：500 mg/kg预处理组肿瘤形成延迟至8周（阴性对照组为4周），肿瘤负荷呈剂量依赖性变化，第60天时阴性对照组与100 mg/kg组出现显著高负荷（p<0.001）。机制研究表明，NF-κB信号通路中TNF-α与p65亚基表达呈剂量依赖性显著降低，500 mg/kg组较阴性对照组差异极显著（p<0.001），且预处理组效果显著优于同步给药组。本实验证实墨西哥蓟叶在小鼠模型中具有明确的癌症预防活性，为后续分子机制研究提供了新方向。

原文链接：

Cancer-Preventive Activity ofArgemone mexicanaLinn Leaves and Its Effect on TNF-α and NF-κB Signalling