The impact ofCandidasp. in the development of oral cancer remains uncertain and requires sensitive analytical approaches for clarification. Given the invasive capabilities of these microorganisms in penetrating and invading host tissues through hyphal invasion, this study sought to detect the presence of fiveCandidasp. in oral biopsy tissue samples from non-smoker patients. Samples were obtained from patients at varying stages of oral carcinogenesis, including dysplasia, carcinoma in situ, OSCC, and histologically benign lesions, and analyzed using Real-Time PCR. Oral tissue samples from 80 patients (46 males and 34 females) were included. Significantly higherC. albicanspresence was detected in the mild/moderate dysplasia group compared to the healthy (p= 0.001), carcinoma in situ (p= 0.031) and OSCC groups (p= 0.000). Similarly,C. tropicaliscarriage was higher in tissues with mild/moderate dysplasia compared to healthy (p= 0.004) and carcinoma in situ (p= 0.019). Our results showed a significant increase in the presence ofC. albicansandC. tropicaliswithin the mild/moderate dysplasia group compared to other cohorts. Coexistence of these two microorganisms was observed, suggesting a potential transition from a commensal state to an opportunistic pathogen, which could be particularly linked to the onset of oral neoplasia.
念珠菌属在口腔癌发展过程中的影响尚不明确，需采用灵敏的分析方法加以阐明。鉴于这类微生物通过菌丝侵袭穿透并侵入宿主组织的能力，本研究旨在从不吸烟患者的口腔活检组织样本中检测五种念珠菌属的存在。样本取自处于不同口腔癌变阶段的患者，包括异型增生、原位癌、口腔鳞状细胞癌及组织学良性病变，并采用实时荧光定量PCR进行分析。研究共纳入80例患者（46例男性，34例女性）的口腔组织样本。结果显示，与健康组（p=0.001）、原位癌组（p=0.031）和口腔鳞状细胞癌组（p=0.000）相比，轻度/中度异型增生组中白色念珠菌的检出率显著更高。同样，热带念珠菌在轻度/中度异型增生组织中的携带率也高于健康组（p=0.004）和原位癌组（p=0.019）。我们的研究结果表明，与其他组别相比，轻度/中度异型增生组中白色念珠菌和热带念珠菌的存在显著增加。观察到这两种微生物的共存现象，提示其可能从共生状态转变为机会性病原体，这一转变可能与口腔肿瘤的发生存在特定关联。
肿瘤(癌症)患者之家