Macrophages play an important role in the suppression and activation of immune anti-cancer response, but little is known about dominant macrophage phenotype in the lung cancer environment, evaluated by bronchoalveolar lavage fluid (BALF). The aim of this study was to characterize macrophages in BALF from a lung affected by cancer (cBALF) and a healthy lung (hBALF) of the same patient regarding their individual macrophage polarization and selected cytokines profile. A total of 36 patients with confirmed lung cancer were investigated. Macrophages markers: CD206 CD163 CD80 CD86 CD40 CD45, Arginase-1, and CD68 were evaluated by flow cytometry. Cytokines (IL-1 RA, IL-6, IL-10, TNF-α, IL-1β, IL-12, IL-23, and TGF-β) profile was analyzed. There was higher median proportion of macrophages in Cbalf than in Hbalf. The population of macrophages presented immunophenotype: Ccd68+brightCD206+brightCD163+brightCD80+ CD86+ CD40+brightCD45+ cArginase+. We observed some trends in the expression of the analyzed antigens in clBALF and hlBLAF. The highest concentrations of IL-1RA and IL-6 were in Cbalf and Hbalf supernatant. There were the correlations between pro- and anti-inflammatory cytokines. The findings showed that macrophages include a diverse and plastic group with the presence of different antigens and cytokines, and determining the target phenotype is a complex and variable process.
巨噬细胞在免疫抗癌反应的抑制与激活中扮演重要角色，然而通过支气管肺泡灌洗液评估的肺癌环境中主导性巨噬细胞表型尚不明确。本研究旨在通过分析同一患者肺癌受累肺组织灌洗液与健康肺组织灌洗液中巨噬细胞的极化特征及特定细胞因子谱，揭示其表型特征。研究共纳入36例确诊肺癌患者，采用流式细胞术检测巨噬细胞标志物（CD206、CD163、CD80、CD86、CD40、CD45、精氨酸酶-1及CD68），并分析细胞因子谱（IL-1RA、IL-6、IL-10、TNF-α、IL-1β、IL-12、IL-23和TGF-β）。结果显示肺癌灌洗液中巨噬细胞中位比例显著高于健康灌洗液。巨噬细胞群体呈现CD68⁺强阳性、CD206⁺强阳性、CD163⁺强阳性、CD80⁺、CD86⁺、CD40⁺强阳性、CD45⁺及精氨酸酶⁺的免疫表型特征。在肺癌与健康灌洗液中观察到部分抗原表达趋势差异，其中IL-1RA与IL-6在两组灌洗液上清中浓度最高。促炎与抗炎细胞因子间存在相关性。研究表明巨噬细胞是具有异质性和可塑性的群体，其抗原与细胞因子表达呈现多样性，确定靶向表型是一个复杂且动态变化的过程。
肿瘤(癌症)患者之家