文章：

早期肠息肉患者黏膜微生物组：基于短读长与长读长16S rRNA测序的解析

Mucosal Microbiome in Patients with Early Bowel Polyps: Inferences from Short-Read and Long-Read 16S rRNA Sequencing

原文发布日期：19 October 2023

DOI: 10.3390/cancers15205045

类型: Article

开放获取: 是

英文摘要：

Numerous studies have correlated dysbiosis in stool microbiota with colorectal cancer (CRC); however, fewer studies have investigated the mucosal microbiome in pre-cancerous bowel polyps. The short-read sequencing of variable regions in the 16S rRNA gene has commonly been used to infer bacterial taxonomy, and this has led, in part, to inconsistent findings between studies. Here, we examined mucosal microbiota from patients who presented with one or more polyps, compared to patients with no polyps, at the time of colonoscopy. We evaluated the results obtained using both short-read and PacBio long-read 16S rRNA sequencing. Neither sequencing technology identified significant differences in microbial diversity measures between patients with or without bowel polyps. Differential abundance measures showed that amplicon sequence variants (ASVs) associated withRuminococcus gnavusandEscherichia coliwere elevated in mucosa from polyp patients, while ASVs associated withParabacteroides merdae,Veillonella nakazawae, andSutterella wadsworthensiswere relatively decreased. OnlyR. gnavuswas consistently identified using both sequencing technologies as being altered between patients with polyps compared to patients without polyps, suggesting differences in technologies and bioinformatics processing impact study findings. Several of the differentially abundant bacteria identified using either sequencing technology are associated with inflammatory bowel diseases despite these patients being excluded from the current study, which suggests that early bowel neoplasia may be associated with a local inflammatory niche.

摘要翻译： 

多项研究已证实粪便微生物群失调与结直肠癌（CRC）之间存在关联；然而，针对癌前肠息肉黏膜微生物组的研究相对较少。目前常用16S rRNA基因可变区的短读长测序技术推断细菌分类学，这在一定程度上导致了不同研究结果的不一致。本研究通过结肠镜检查，对比了存在一处或多处息肉的患者与无息肉患者的黏膜微生物群，并评估了短读长测序与PacBio长读长16S rRNA测序的结果。两种测序技术均未发现息肉患者与非息肉患者在微生物多样性指标上存在显著差异。差异丰度分析显示，息肉患者黏膜中与瘤胃球菌属（Ruminococcus gnavus）和大肠杆菌（Escherichia coli）相关的扩增子序列变体（ASVs）水平升高，而与粪副拟杆菌（Parabacteroides merdae）、中泽韦荣球菌（Veillonella nakazawae）及沃氏萨特菌（Sutterella wadsworthensis）相关的ASVs相对减少。仅瘤胃球菌属在两种测序技术中均被一致鉴定为息肉患者与非息肉患者间的差异菌群，这表明测序技术和生物信息学处理的差异会影响研究结果。尽管本研究已排除炎症性肠病患者，但通过两种测序技术鉴定的部分差异丰度细菌均与炎症性肠病相关，提示早期肠道肿瘤形成可能与局部炎症微环境存在关联。

原文链接：

Mucosal Microbiome in Patients with Early Bowel Polyps: Inferences from Short-Read and Long-Read 16S rRNA Sequencing