Colorectal cancer (CRC) is a significant global health concern. Microbial dysbiosis and associated metabolites have been associated with CRC occurrence and progression. This study aims to analyze the gut microbiota composition and the enriched metabolic pathways in patients with late-stage CRC. In this study, a cohort of 25 CRC patients diagnosed at late stage III and IV and 25 healthy participants were enrolled. The fecal bacterial composition was investigated using V3-V4 ribosomal RNA gene sequencing, followed by clustering and linear discriminant analysis (LDA) effect size (LEfSe) analyses. A cluster of ortholog genes’ (COG) functional annotations and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were employed to identify enrichment pathways between the two groups. The findings showed that the fecal microbiota between the two groups varied significantly in alpha and beta diversities. CRC patients’ fecal samples had significantly enriched populations ofStreptococcus salivarius,S. parasanguins,S. anginosus,Lactobacillus mucosae,L. gasseri,Peptostreptococcus,Eubacterium,Aerococcus, Family XIII_AD3001 Group,Erysipelatoclostridium,Escherichia-Shigella,Klebsiella,Enterobacter,Alistipes,Ralstonia, andPseudomonas(Q < 0.05). The enriched pathways identified in the CRC group were amino acid transport, signaling and metabolism, membrane biogenesis, DNA replication and mismatch repair system, and protease activity (Q < 0.05). These results suggested that the imbalance between intestinal bacteria and the elevated level of the predicated functions and pathways may contribute to the development of advanced CRC tumors. Further research is warranted to elucidate the exact role of the gut microbiome in CRC and its potential implications for use in diagnostic, prevention, and treatment strategies.
结直肠癌是全球范围内重要的健康问题。微生物菌群失调及其相关代谢产物与结直肠癌的发生发展密切相关。本研究旨在分析晚期结直肠癌患者的肠道菌群组成及其富集的代谢通路。研究纳入25例III-IV期晚期结直肠癌患者及25名健康对照者，采用V3-V4区核糖体RNA基因测序技术分析粪便菌群组成，并进行聚类分析和线性判别分析效应量分析。通过直系同源基因簇功能注释和京都基因与基因组百科全书数据库鉴定两组间的富集通路。结果显示两组粪便菌群在α和β多样性方面存在显著差异。结直肠癌患者粪便样本中唾液链球菌、副血链球菌、咽峡炎链球菌、黏膜乳杆菌、加氏乳杆菌、消化链球菌属、真杆菌属、气球菌属、XIII_AD3001菌科、丹毒丝菌属、埃希氏菌-志贺氏菌属、克雷伯氏菌属、肠杆菌属、另枝菌属、罗尔斯通氏菌属和假单胞菌属的丰度显著富集（Q < 0.05）。结直肠癌组富集的通路包括氨基酸转运与信号转导代谢、膜生物合成、DNA复制与错配修复系统以及蛋白酶活性（Q < 0.05）。这些结果表明肠道菌群失衡及其预测功能和通路的水平升高可能促进晚期结直肠肿瘤的发展。需要进一步研究阐明肠道微生物组在结直肠癌中的确切作用及其在诊断、预防和治疗策略中的潜在应用价值。
Identification of Gut Microbiota Profile Associated with Colorectal Cancer in Saudi Population
肿瘤(癌症)患者之家