Colon tumors have a very complicated and poorly understood pathogenesis. Plant-based organic compounds might provide a novel source for cancer treatment with a sufficient novel mode of action. The objective of this study was to analyze and evaluate the efficacy ofAloe secundiflora’s(AS) methanolic extracts on the expression ofCASPS9,5-LOX,Bcl2,Bcl-xL, andCOX-2in colorectal cancer (CRC) management. Caco-2 cell lines were used in the experimental study. In the serial exhaustive extraction (SEE) method, methanol was utilized as the extraction solvent. Upon treatment ofCASPS9with the methanolic extracts, the expression of the genes was progressively upregulated, thus, dose-dependently increasing the rate of apoptosis. On the other hand, the expressions of5-LOX,Bcl2, andBcl-xLwere variably downregulated in a dose-dependent manner. This is a unique novel study that evaluated the effects of AS methanolic extracts in vitro on CRC cell lines using different dosage concentrations. We, therefore, recommend the utilization of AS and the application of methanol as the extraction solvent of choice for maximum modulatory benefits in CRC management. In addition, we suggest research on the specific metabolites in AS involved in the modulatory pathways that suppress the development of CRC and potential metastases.
结肠肿瘤的发病机制极为复杂且尚未被充分阐明。植物来源的有机化合物可能通过其独特的作用机制，为癌症治疗提供新型药物来源。本研究旨在分析和评估芦荟（Aloe secundiflora）甲醇提取物在结直肠癌治疗中对CASPS9、5-LOX、Bcl2、Bcl-xL及COX-2基因表达的调控作用。实验采用Caco-2细胞系，通过连续彻底提取法，以甲醇作为提取溶剂。研究结果显示：经甲醇提取物处理后，CASPS9基因表达呈剂量依赖性上调，从而促进细胞凋亡；而5-LOX、Bcl2和Bcl-xL基因表达则呈现不同程度的剂量依赖性下调。本研究首次通过不同浓度梯度，在体外评估了芦荟甲醇提取物对结直肠癌细胞系的影响。基于以上发现，我们建议在结直肠癌治疗中采用芦荟提取物，并优先选用甲醇作为提取溶剂，以实现最佳的调控效果。此外，建议进一步研究芦荟中参与抑制结直肠癌发生及潜在转移调控通路的具体代谢产物。
肿瘤(癌症)患者之家