Optimizing cell culture conditions is essential to ensure experimental reproducibility. To improve the accuracy of preclinical predictions about the response of tumor cells to different classes of drugs, researchers have used 2D or 3D cell cultures in vitro to mimic the cellular processes occurring in vivo. While 2D cell culture provides valuable information on how therapeutic agents act on tumor cells, it cannot quantify how the tumor microenvironment influences the response to therapy. This review presents the necessary strategies for transitioning from 2D to 3D cell cultures, which have facilitated the rapid evolution of bioengineering techniques, leading to the development of microfluidic technology, including organ-on-chip and tumor-on-chip devices. Additionally, the study aims to highlight the impact of the advent of 3D bioprinting and microfluidic technology and their implications for improving cancer treatment and approaching personalized therapy, especially for lung cancer. Furthermore, implementing microfluidic technology in cancer studies can generate a series of challenges and future perspectives that lead to the discovery of new predictive markers or targets for antitumor treatment.
优化细胞培养条件对于确保实验可重复性至关重要。为提高肿瘤细胞对不同类别药物反应的临床前预测准确性，研究人员采用二维或三维体外细胞培养模型来模拟体内细胞过程。尽管二维细胞培养能够提供治疗药物如何作用于肿瘤细胞的重要信息，但无法量化肿瘤微环境对治疗反应的影响。本综述阐述了从二维向三维细胞培养转型的必要策略，这些策略推动了生物工程技术的快速发展，促进了微流控技术的进步，包括器官芯片和肿瘤芯片装置的研发。此外，本研究旨在强调三维生物打印与微流控技术的出现对改善癌症治疗及实现个性化疗法（特别是针对肺癌）的影响。在癌症研究中应用微流控技术将带来一系列挑战与未来展望，有望发现新的抗肿瘤治疗预测标志物或靶点。
Cell Culture Model Evolution and Its Impact on Improving Therapy Efficiency in Lung Cancer
肿瘤(癌症)患者之家