Interleukin-8 (IL-8), a pro-inflammatory cytokine, is upregulated in CRC and plays an important role in its development and progression. Genetic variants in theIL-8gene may impact the risk of CRC by modulating IL-8 levels. Our primary objective was to investigate the role ofIL-8genotypes in the development of CRC. To accomplish this, we employed the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to analyze the genotypes ofIL-8rs4017, rs2227306, rs2227543, and rs1126647 in 362 CRC patients and 362 controls. Additionally, we evaluated the interactions between these genotypes and factors such as age, gender, smoking, alcohol consumption, and body mass index (BMI) status in relation to the risk of CRC. Furthermore, we utilized quantitative reverse transcription-PCR to measure the serum IL-8. The results demonstrated a significant difference in the distribution of rs4017 genotypes between the control and case groups (pfor trend = 0.0059). Logistic regression analysis revealed that individuals with variant AA genotype had a 1.92-fold higher CRC risk (95% confidence interval [CI] = 1.28–2.89,p= 0.0023). Moreover, carriers of theIL-8rs4017 AT + AA genotypes exhibited a significant association with CRC risk (odds ratio [OR] = 1.39, 95% CI = 1.02–1.91,p= 0.0460). Additionally, individuals withIL-8rs4017 AA genotype displayed significantly elevated serum IL-8 compared to those with TT genotype at a 1.73–fold level (p< 0.0001), indicating a correlation between genotype and phenotype. In conclusion, the genotypes ofIL-8rs4017, along with their associated expression levels, can potentially serve as predictive markers for the risk of CRC.
白细胞介素-8（IL-8）作为一种促炎细胞因子，在结直肠癌（CRC）中表达上调，并在其发生发展中发挥重要作用。IL-8基因的遗传变异可能通过调节IL-8水平影响CRC的患病风险。本研究主要目的是探讨IL-8基因型在CRC发生中的作用。为此，我们采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对362例CRC患者和362例对照者的IL-8基因rs4017、rs2227306、rs2227543和rs1126647位点基因型进行分析。同时，评估了这些基因型与年龄、性别、吸烟、饮酒及体重指数（BMI）状态等因素在CRC风险中的交互作用。此外，采用定量逆转录PCR检测血清IL-8水平。结果显示，对照组与病例组之间rs4017基因型分布存在显著差异（趋势p值=0.0059）。逻辑回归分析表明，携带变异AA基因型的个体CRC风险增加1.92倍（95%置信区间[CI]=1.28–2.89，p=0.0023）。此外，携带IL-8 rs4017 AT+AA基因型者与CRC风险显著相关（比值比[OR]=1.39，95% CI=1.02–1.91，p=0.0460）。值得注意的是，与TT基因型携带者相比，IL-8 rs4017 AA基因型携带者的血清IL-8水平显著升高1.73倍（p<0.0001），表明基因型与表型存在相关性。综上所述，IL-8 rs4017基因型及其相关表达水平可能作为预测CRC风险的生物标志物。
The Significant Impacts of Interleukin-8 Genotypes on the Risk of Colorectal Cancer in Taiwan
肿瘤(癌症)患者之家