Breast cancer (BC) is the leading cause of cancer mortality among women in Ethiopia. Overall, women of African ancestry have the highest death toll due to BC compared to other racial/ethnic groups. The cause of the disparity in mortality is unclear. Recently, studies conducted in the United States and other high-income countries highlighted the role of microbial dysbiosis in BC initiation, tumor growth, and treatment outcome. However, the extent to which inter-individual differences in the makeup of microbiota are associated with clinical and histopathological outcomes in Ethiopian women has not been studied. The goal of our study was to profile the microbiome in breast tumor and normal adjacent to tumor (NAT) tissues of the same donor and to identify associations between microbial composition and abundance and clinicopathological factors in Ethiopian women with BC. We identified 14 microbiota genera in breast tumor tissues that were distinct from NAT tissues, of whichSphingobium,Anaerococcus,Corynebacterium,Delftia, andEnhydrobacterwere most significantly decreased in breast tumors compared to NAT tissues. Several microbial genera significantly differed by clinicopathological factors in Ethiopian women with BC. Specifically, the genusBurkholderiamore strongly correlated with aggressive triple negative (TNBC) and basal-like breast tumors. The generaAlkanindiges,Anoxybacillus,Leifsonia, andExiguobacteriummost strongly correlated with HER2-E tumors. Luminal A and luminal B tumors also correlated withAnoxybacillusbut not as strongly as HER2−E tumors. A relatively higher abundance of the genusCitrobactermost significantly correlated with advanced-stage breast tumors compared to early-stage tumors. This is the first study to report an association between breast microbial dysbiosis and clinicopathological factors in Ethiopian women.
乳腺癌是埃塞俄比亚女性癌症死亡的首要原因。总体而言，与其他种族/族裔群体相比，非洲裔女性因乳腺癌导致的死亡率最高。这种死亡率差异的原因尚不明确。近期在美国及其他高收入国家开展的研究揭示了微生物菌群失调在乳腺癌发生、肿瘤生长及治疗结果中的作用。然而，埃塞俄比亚女性个体间微生物组成差异与临床及组织病理学结果之间的关联程度尚未得到研究。本研究旨在分析同一供体乳腺癌组织与癌旁正常组织中的微生物组特征，并识别埃塞俄比亚乳腺癌患者微生物组成及丰度与临床病理因素之间的关联。我们在乳腺癌组织中鉴定出14个与癌旁正常组织存在差异的微生物属，其中鞘氨醇单胞菌属、厌氧球菌属、棒状杆菌属、代尔夫特菌属及水杆菌属在乳腺癌组织中的丰度较癌旁正常组织显著降低。多个微生物属在埃塞俄比亚乳腺癌患者的临床病理特征中呈现显著差异。具体而言，伯克霍尔德菌属与侵袭性三阴性乳腺癌及基底样乳腺癌肿瘤呈现更强相关性；烷烃降解菌属、厌氧芽孢杆菌属、莱夫森氏菌属及微小杆菌属与HER2富集型肿瘤关联最为显著；管腔A型与管腔B型肿瘤虽也与厌氧芽孢杆菌属相关，但其关联强度弱于HER2富集型肿瘤。与早期乳腺癌相比，柠檬酸杆菌属相对较高的丰度与晚期乳腺癌肿瘤呈最显著相关。本研究首次报道了埃塞俄比亚女性乳腺微生物失调与临床病理因素之间的关联。
肿瘤(癌症)患者之家