文章：

三维体外肿瘤球体模型在抗癌疗法评估中的应用：最新进展

Three-Dimensional In Vitro Tumor Spheroid Models for Evaluation of Anticancer Therapy: Recent Updates

原文发布日期：4 October 2023

DOI: 10.3390/cancers15194846

类型: Article

开放获取: 是

英文摘要：

Advanced tissue engineering processes and regenerative medicine provide modern strategies for fabricating 3D spheroids. Several different 3D cancer models are being developed to study a variety of cancers. Three-dimensional spheroids can correctly replicate some features of solid tumors (such as the secretion of soluble mediators, drug resistance mechanisms, gene expression patterns and physiological responses) better than 2D cell cultures or animal models. Tumor spheroids are also helpful for precisely reproducing the three-dimensional organization and microenvironmental factors of tumors. Because of these unique properties, the potential of 3D cell aggregates has been emphasized, and they have been utilized in in vitro models for the detection of novel anticancer drugs. This review discusses applications of 3D spheroid models in nuclear medicine for diagnosis and therapy, immunotherapy, and stem cell and photodynamic therapy and also discusses the establishment of the anticancer activity of nanocarriers.

摘要翻译： 

先进的组织工程学与再生医学技术为三维球体的构建提供了现代策略。目前多种三维癌症模型正在开发中，用于研究各类癌症。相较于二维细胞培养或动物模型，三维球体能够更准确地模拟实体瘤的某些特征（如可溶性介质分泌、耐药机制、基因表达模式和生理反应）。肿瘤球体还有助于精确再现肿瘤的三维组织结构及微环境因素。基于这些独特性质，三维细胞聚集体的潜力日益凸显，已被广泛应用于新型抗癌药物的体外筛选模型。本综述探讨了三维球体模型在核医学诊断治疗、免疫疗法、干细胞与光动力疗法中的应用，并对纳米载体抗癌活性的评估体系进行了论述。

原文链接：

Three-Dimensional In Vitro Tumor Spheroid Models for Evaluation of Anticancer Therapy: Recent Updates