The presence of checkpoint markers in cancer cells aids in immune escape. The identification of checkpoint markers and early cancer markers is of utmost importance to gain clarity regarding the relationship between colitis and progressive inflammation leading to cancer. Herein, the gene expression levels of checkpoint makers, cancer-related pathways, and cancer genes in colon tissues of mouse models of chronic colitis (WinnieandWinnie-Prolapsemice) using next-generation sequencing are determined.Winniemice are a result of aMuc2missense mutation. The identification of such genes and their subsequent expression and role at the protein level would enable novel markers for the early diagnosis of cancer in IBD patients. The differentially expressed genes in the colonic transcriptome were analysed based on the Kyoto Encyclopedia of Genes and Genomes pathway. The expression of several oncogenes is associated with the severity of IBD, withWinnie-Prolapsemice expressing a large number of key genes associated with development of cancer. This research presents a number of new targets to evaluate for the development of biomarkers and therapeutics.
癌细胞中检查点标记物的存在有助于免疫逃逸。识别检查点标记物与早期癌症标志物对于明确结肠炎与进展性炎症导致癌症之间的关系至关重要。本研究采用新一代测序技术，测定慢性结肠炎小鼠模型（Winnie及Winnie-Prolapse小鼠）结肠组织中检查点标记物、癌症相关通路及癌症基因的表达水平。Winnie小鼠由Muc2错义突变产生。识别此类基因及其在蛋白质水平的表达与作用，将为炎症性肠病患者癌症早期诊断提供新型标志物。基于京都基因与基因组百科全书通路分析结肠转录组差异表达基因发现，多个癌基因的表达与炎症性肠病严重程度相关，其中Winnie-Prolapse小鼠大量表达与癌症发生相关的关键基因。本研究为生物标志物和治疗方法的开发提供了多个新的评估靶点。
肿瘤(癌症)患者之家