文章：

采用非偏向性蛋白质组学方法鉴定子宫内膜癌潜在新型分子靶点

Identification of Potentially Novel Molecular Targets of Endometrial Cancer Using a Non-Biased Proteomic Approach

原文发布日期：21 September 2023

DOI: 10.3390/cancers15184665

类型: Article

开放获取: 是

英文摘要：

The present study was aimed at identifying novel proteins in endometrial cancer (EC), employing proteomic analysis of tissues obtained after surgery. A differential MS-based proteomic analysis was conducted from whole tissues dissected from biopsies from post-menopausal women, histologically confirmed as endometrial cancer (two endometrioid and two serous; n = 4) or normal atrophic endometrium (n = 4), providing 888 differentially expressed proteins with 246 of these previously documented elsewhere as expressed in EC and 372 proteins not previously demonstrated to be expressed in EC but associated with other types of cancer. Additionally, 33 proteins not recorded previously in PubMed as being expressed in any forms of cancer were also identified, with only 26 of these proteins having a publication associated with their expression patterns or putative functions. The putative functions of the 26 proteins (GRN, APP, HEXA, CST3, CAD, QARS, SIAE, WARS, MYH8, CLTB, GOLIM4, SCARB2, BOD1L1, C14orf142, C9orf142, CCDC13, CNPY4, FAM169A, HN1L, PIGT, PLCL1, PMFBP1, SARS2, SCPEP1, SLC25A24 and ZC3H4) in other tissues point towards and provide a basis for further investigation of these previously unrecognised novel EC proteins. The developmental biology, disease, extracellular matrix, homeostatic, immune, metabolic (both RNA and protein), programmed cell death, signal transduction, molecular transport, transcriptional networks and as yet uncharacterised pathways indicate that these proteins are potentially involved in endometrial carcinogenesis and thus may be important in EC diagnosis, prognostication and treatment and thus are worthy of further investigation.

摘要翻译： 

本研究旨在通过手术获取组织样本进行蛋白质组学分析，以鉴定子宫内膜癌（EC）中的新型蛋白质。研究对绝经后妇女活检组织中解剖出的完整组织进行了差异性质谱蛋白质组学分析，这些组织经组织学确认为子宫内膜癌（两种子宫内膜样癌和两种浆液性癌；n = 4）或正常萎缩性子宫内膜（n = 4）。分析共鉴定出888种差异表达蛋白质，其中246种此前已有文献记载在EC中表达，372种蛋白质虽未在EC中发现表达，但与其他类型癌症相关。此外，还鉴定出33种此前未在PubMed中记录在任何形式癌症中表达的蛋白质，其中仅有26种蛋白质有与其表达模式或推定功能相关的文献发表。这26种蛋白质（GRN、APP、HEXA、CST3、CAD、QARS、SIAE、WARS、MYH8、CLTB、GOLIM4、SCARB2、BOD1L1、C14orf142、C9orf142、CCDC13、CNPY4、FAM169A、HN1L、PIGT、PLCL1、PMFBP1、SARS2、SCPEP1、SLC25A24和ZC3H4）在其他组织中的推定功能为这些先前未被认识的EC新型蛋白质的进一步研究提供了方向和基础。发育生物学、疾病、细胞外基质、稳态、免疫、代谢（包括RNA和蛋白质）、程序性细胞死亡、信号转导、分子转运、转录网络以及尚未明确的通路表明，这些蛋白质可能参与子宫内膜癌的发生，因此在EC的诊断、预后和治疗中可能具有重要意义，值得进一步研究。

原文链接：

Identification of Potentially Novel Molecular Targets of Endometrial Cancer Using a Non-Biased Proteomic Approach