文章：

胰腺导管腺癌患者特异性膜抗原表达的免疫组织化学评估

Immunohistochemical Evaluation of the Expression of Specific Membrane Antigens in Patients with Pancreatic Ductal Adenocarcinoma

原文发布日期：15 September 2023

DOI: 10.3390/cancers15184586

类型: Article

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英文摘要：

(1) Background: Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignancies. The lack of validated disease biomarkers makes timely diagnosis challenging in most cases. Cell membrane and surface proteins play a crucial role in several routes of oncogenesis. The aim of this study was to evaluate the expression of six membrane antigens on PDAC (CA 19-9, mucin 1 and 4 (MUC1, MUC4), mesothelin (MSLN), Annexin A10 (ANXA10), Glypican-1 (GPC-1)) and their correlation with oncologic outcomes. (2) Methods: Immunohistochemical staining for CA 19.9, MUC1, MUC4, MSLN, ANXA10, and GPC-1 of surgical samples of 50 consecutive patients with PDAC was performed. Antigen expression for tumor, ductal, and acinar tissues was classified according to the histo-score (H-score) by two pathologists. (3) Results: Recurrence rate was 47% and 18 patients (36%) deceased (median follow-up 21.5 months). Immunostaining for CA 19-9 and MUC1 showed a significantly higher expression in the neoplastic tissue compared to non-tumor ductal and acinar tissues (p< 0.001). MUC4, MSLN, ANXA10, and GPC-1 were selectively expressed in the neoplastic tissue (p< 0.001). A CA 19-9 H-score value >270 was independently associated with a worse overall survival (p= 0.05) and disease-free survival (p= 0.05). (4) Conclusions: CA 19-9 and MUC1 are highly expressed in PDAC cells. The histological expression of CA 19-9 may predict prognosis. MUC4, MSLN, ANXA10, and GPC-1 are selectively expressed by neoplastic tissue and may represent a potential histological biomarker of disease.

摘要翻译： 

(1) 背景：胰腺导管腺癌（PDAC）是最具致死性的恶性肿瘤之一。由于缺乏经过验证的疾病生物标志物，在大多数情况下及时诊断面临挑战。细胞膜及表面蛋白在多种致癌途径中发挥关键作用。本研究旨在评估PDAC中六种膜抗原（CA 19-9、黏蛋白1和4（MUC1、MUC4）、间皮素（MSLN）、膜联蛋白A10（ANXA10）、磷脂酰肌醇蛋白聚糖-1（GPC-1））的表达及其与肿瘤学预后的相关性。(2) 方法：对50例连续收治的PDAC患者手术样本进行CA 19-9、MUC1、MUC4、MSLN、ANXA10和GPC-1的免疫组织化学染色。由两名病理学家根据组织学评分（H-score）对肿瘤组织、导管组织和腺泡组织的抗原表达进行分类评估。(3) 结果：复发率为47%，18例患者（36%）死亡（中位随访时间21.5个月）。CA 19-9和MUC1在肿瘤组织中的免疫染色表达显著高于非肿瘤性导管及腺泡组织（p<0.001）。MUC4、MSLN、ANXA10和GPC-1在肿瘤组织中呈现选择性表达（p<0.001）。CA 19-9 H-score值>270与较差的总生存期（p=0.05）和无病生存期（p=0.05）独立相关。(4) 结论：CA 19-9和MUC1在PDAC细胞中高表达。CA 19-9的组织学表达可能具有预后预测价值。MUC4、MSLN、ANXA10和GPC-1在肿瘤组织中特异性表达，可能成为潜在的疾病组织学生物标志物。

原文链接：

Immunohistochemical Evaluation of the Expression of Specific Membrane Antigens in Patients with Pancreatic Ductal Adenocarcinoma