The dual role of necroptosis in inhibiting and promoting tumor development has gradually received much attention because of its essential significance for targeted treatment. Accordingly, this study aims to explore the relationship between necroptosis and oral squamous cell carcinoma (OSCC), and search for novel prognostic factors for OSCC. RNA-seq data and clinical information were downloaded from TCGA and GTEx databases. The prognostic signature of necroptosis-related genes (NRGs) was constructed by univariate Cox regression analysis and the LASSO Cox regression model. Moreover, survival analyses, ROC curves, and nomograms were adopted to further analyze. GO and KEGG analyses and immune infiltration analyses were used for function enrichment and immune feature research in turn. The NRG prognostic signature expression was higher in OSCC tissues than in normal tissues, and the overall survival (OS) rate of the high-expression group was much lower. HPRT1 was proved to be an independent prognostic factor in OSCC. Furthermore, the function enrichment analyses revealed that NRGs were involved in necroptosis, apoptosis, inflammation, and immune reaction. The expression of NRGs was related to immunosuppression in OSCC. Furthermore, the knockdown of HPRT1 could suppress the proliferation and migration of OSCC. In conclusion, the high expression of NRG prognostic signature is associated with poor prognosis in OSCC, and HPRT1 can serve as a novel independent prognostic factor for OSCC.
坏死性凋亡在抑制和促进肿瘤发展中的双重作用因其对靶向治疗的重要意义而逐渐受到广泛关注。本研究旨在探讨坏死性凋亡与口腔鳞状细胞癌（OSCC）的关系，并寻找新的OSCC预后因子。从TCGA和GTEx数据库获取RNA-seq数据及临床信息，通过单因素Cox回归分析和LASSO Cox回归模型构建坏死性凋亡相关基因（NRGs）的预后特征模型。采用生存分析、ROC曲线和列线图进行深入分析，并依次通过GO与KEGG分析、免疫浸润分析进行功能富集和免疫特征研究。结果显示，NRG预后特征在OSCC组织中的表达高于正常组织，高表达组的总生存率显著降低。HPRT1被证实是OSCC的独立预后因子。功能富集分析表明NRGs参与坏死性凋亡、细胞凋亡、炎症及免疫反应过程，其表达与OSCC的免疫抑制状态相关。进一步实验证实敲低HPRT1可抑制OSCC的增殖与迁移。综上所述，NRG预后特征的高表达与OSCC不良预后相关，HPRT1可作为OSCC的新型独立预后因子。
Prognostic Value of Necroptosis-Related Genes Signature in Oral Squamous Cell Carcinoma
肿瘤(癌症)患者之家