文章：

十二指肠液代谢组学在胆道癌诊断中的应用

Metabolomics of Duodenal Juice for Biliary Tract Cancer Diagnosis

原文发布日期：1 September 2023

DOI: 10.3390/cancers15174370

类型: Article

开放获取: 是

英文摘要：

The poor prognosis of malignant biliary diseases is partially caused by their difficult early diagnosis. Therefore, many patients are only diagnosed at advanced stages. This study aimed to improve diagnosis by clarifying the differences in the duodenal juice metabolomes of benign and malignant biliary diseases. From October 2021 to January 2023, duodenal juice was obtained from 67 patients with suspected biliary diseases who required endoscopic ultrasonography and endoscopic retrograde cholangiography for diagnosis/treatment. The samples metabolomes were analyzed via nuclear magnet resonance spectroscopy using an 800-MHz spectrometer. Metabolomes of malignant and benign diseases were then compared, and multivariate analysis was performed to determine the relevant factors for malignancy/benignancy. For benignancy, no significant predictors were observed. For malignancy, acetone was a significant predictor, with higher concentrations in the malignant group than in the benign group. Regarding the receiver operating characteristic curve analysis for biliary tract carcinoma diagnosis, the predictive value of acetone in duodenal juice was comparable with serum CA19-9 levels (area under the curve: 0.7330 vs. 0.691,p= 0.697). In conclusion, duodenal juice metabolomics is a feasible method that is available for differential diagnosis in the biliary disease field.

摘要翻译： 

恶性胆道疾病预后不良，部分原因在于其早期诊断困难，导致许多患者确诊时已处于晚期。本研究旨在通过阐明良恶性胆道疾病患者十二指肠液代谢组的差异来改善诊断。2021年10月至2023年1月期间，从67例疑似胆道疾病且需行超声内镜及内镜逆行胰胆管造影以明确诊断/治疗的患者中获取十二指肠液样本。使用800兆赫兹核磁共振波谱仪对样本代谢组进行分析，比较良恶性疾病的代谢组特征，并通过多变量分析确定与恶性/良性相关的因素。对于良性病变，未观察到显著预测因子；而对于恶性病变，丙酮是重要预测因子，其在恶性组中的浓度显著高于良性组。在胆道癌诊断的受试者工作特征曲线分析中，十二指肠液中丙酮的预测价值与血清CA19-9水平相当（曲线下面积：0.7330对比0.691，p=0.697）。综上所述，十二指肠液代谢组学是一种可行的检测方法，可用于胆道疾病领域的鉴别诊断。

原文链接：

Metabolomics of Duodenal Juice for Biliary Tract Cancer Diagnosis