Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology has transformed oncology research in many ways. Breast cancer is the most prevalent malignancy globally and triple negative breast cancer (TNBC) is one of the most aggressive subtypes with numerous challenges still to be faced. In this work, we have explained what CRISPR consists of and listed its applications in breast cancer while focusing on TNBC research. These are disease modelling, the search for novel genes involved in tumour progression, sensitivity to drugs and immunotherapy response, tumour fitness, diagnosis, and treatment. Additionally, we have listed the current delivery methods employed for the delivery of CRISPR systems in vivo. Lastly, we have highlighted the limitations that CRISPR technology is subject to and the future directions that we envisage. Overall, we have provided a round summary of the aspects concerning CRISPR in breast cancer/TNBC research.
成簇规律间隔短回文重复序列（CRISPR）技术已在多方面革新了肿瘤学研究。乳腺癌是全球发病率最高的恶性肿瘤，其中三阴性乳腺癌（TNBC）作为最具侵袭性的亚型之一，仍面临诸多挑战。本文系统阐述了CRISPR技术的构成原理，重点聚焦TNBC研究领域，列举了该技术在乳腺癌研究中的具体应用方向，包括疾病模型构建、肿瘤进展相关新基因发掘、药物敏感性及免疫治疗反应研究、肿瘤适应性分析、诊断与治疗策略开发。同时，我们梳理了目前CRISPR系统在体内递送所采用的主要技术方法。最后，本文着重探讨了CRISPR技术当前存在的局限性，并展望了未来发展方向。总体而言，本研究为CRISPR技术在乳腺癌/三阴性乳腺癌研究领域的应用提供了全面综述。
Applications of CRISPR Technology to Breast Cancer and Triple Negative Breast Cancer Research
肿瘤(癌症)患者之家