Bladder cancer (BC) is the 6th most common cancer worldwide, with tobacco smoking considered as its main risk factor. Accumulating evidence has found associations between genetic variants and the risk of BC. Candidate gene-environment interaction studies have suggested interactions between cigarette smoking andNAT2/GSTM1gene variants. Our objective was to perform a genome-wide association case-only study using the French national prospective COBLAnCE cohort (COhort to study BLAdder CancEr), focusing on smoking behavior. The COBLAnCE cohort comprises 1800 BC patients enrolled between 2012 and 2018. Peripheral blood samples collected at enrolment were genotyped using the Illumina Global Screening Array with a Multi-Disease drop-in panel. Genotyping data (9,719,614 single nucleotide polymorphisms (SNP)) of 1674, 1283, and 1342 patients were analyzed for smoking status, average tobacco consumption, and age at smoking initiation, respectively. A genome-wide association study (GWAS) was conducted adjusting for gender, age, and genetic principal components. The results suggest new candidate loci (4q22.1, 12p13.1, 16p13.3) interacting with smoking behavior for the risk of BC. Our results need to be validated in other case-control or cohort studies.
膀胱癌是全球第六大常见癌症，吸烟被认为是其主要危险因素。越来越多的证据发现遗传变异与膀胱癌风险之间存在关联。候选基因-环境交互作用研究表明，吸烟与NAT2/GSTM1基因变异之间存在交互作用。我们的目标是利用法国国家前瞻性COBLAnCE队列（膀胱癌研究队列），以吸烟行为为重点，进行一项全基因组关联病例研究。COBLAnCE队列包括2012年至2018年间招募的1800名膀胱癌患者。入组时收集的外周血样本使用Illumina Global Screening Array和多疾病插入面板进行基因分型。分别对1674名、1283名和1342名患者的基因分型数据（9,719,614个单核苷酸多态性）进行了吸烟状况、平均烟草消费量和开始吸烟年龄的分析。全基因组关联研究（GWAS）在调整了性别、年龄和遗传主成分后进行。结果表明，新的候选位点（4q22.1、12p13.1、16p13.3）与吸烟行为相互作用，影响膀胱癌风险。我们的结果需要在其他病例对照或队列研究中得到验证。
肿瘤(癌症)患者之家