Background: Advancements in DNA sequencing technology have facilitated the assessment of the connection between the oral microbiome and various diseases. The aim of the present study was to investigate the salivary microbiota composition employing for the first time in the literature the Oxford Nanopore Technology in patients affected by oral squamous cell carcinoma (OSCC). Methods: Unstimulated saliva samples of 31 patients were collected (24 OSCC patients and 7 controls). DNA was extracted using the QIAamp DNA Blood Kit and metagenomic long sequencing reads were performed using the MinION device. Results: In the OSCC group, 13 were males and 11 were females, with a mean age of 65.5 ± 13.9 years; in the control group, 5 were males and 2 were females, with a mean age of 51.4 ± 19.2 years. The border of the tongue was the most affected OSCC site. The microorganisms predominantly detected in OSCC patients werePrevotella,Chlamydia,Tissierellia,Calothrix,Leotiomycetes,FirmicutesandZetaproteobacteria. Conclusions: This study confirmed the predominance of periodontopathic bacteria in the salivary microbiome in the OSCC group. If a direct correlation between oral dysbiosis and OSCC onset was proven, it could lead to new prevention strategies and early diagnostic tools.
背景：DNA测序技术的进步促进了口腔微生物组与多种疾病关联性的评估。本研究旨在首次采用牛津纳米孔技术，探究口腔鳞状细胞癌（OSCC）患者唾液微生物群的组成。方法：收集31例患者（24例OSCC患者和7例对照）的非刺激性唾液样本。使用QIAamp DNA Blood Kit提取DNA，并通过MinION设备进行宏基因组长读长测序。结果：OSCC组中男性13例、女性11例，平均年龄65.5±13.9岁；对照组中男性5例、女性2例，平均年龄51.4±19.2岁。舌缘是最常见的OSCC发病部位。在OSCC患者中主要检测到的微生物包括普雷沃菌属、衣原体属、蒂西耶菌属、眉藻属、锤舌菌纲、厚壁菌门和泽塔变形菌纲。结论：本研究证实了OSCC组唾液微生物组中牙周致病菌占主导地位。若口腔菌群失调与OSCC发病之间的直接关联得到证实，或将催生新的预防策略和早期诊断工具。
肿瘤(癌症)患者之家