文章：

癌症女童冷冻卵巢组织中未来应用所需的最小浸润性疾病识别：一项系统性综述

Minimal Infiltrative Disease Identification in Cryopreserved Ovarian Tissue of Girls with Cancer for Future Use: A Systematic Review

原文发布日期：22 August 2023

DOI: 10.3390/cancers15174199

类型: Article

开放获取: 是

英文摘要：

Background: Ovarian tissue cryopreservation and transplantation are the only available fertility techniques for prepubertal girls with cancer. Though autotransplantation carries a risk of reintroducing malignant cells, it can be avoided by identifying minimal infiltrative disease (MID) within ovarian tissue. Methods: A broad search for peer-reviewed articles in the PubMed database was conducted in accordance with PRISMA guidelines up to March 2023. Search terms included ‘minimal residual disease’, ‘cryopreservation’, ‘ovarian’, ‘cancer’ and synonyms. Results: Out of 542 identified records, 17 were included. Ovarian tissues of at least 115 girls were evaluated and categorized as: hematological malignancies (n= 56; 48.7%), solid tumors (n= 42; 36.5%) and tumors of the central nervous system (n= 17; 14.8%). In ovarian tissue of 25 patients (21.7%), MID was detected using RT-qPCR, FISH or multicolor flow cytometry: 16 of them (64%) being ALL (IgHrearrangements with/withoutTRG,BCL-ABL1,EA2-PBX1,TEL-AML1fusion transcripts), 3 (12%) Ewing sarcoma (EWS-FLI1fusion transcript,EWSR1rearrangements), 3 (12%) CML (BCR-ABL1fusion transcript,FLT3) and 3 (12%) AML (leukemia-associated immunophenotypes,BCR-ABL1fusion transcript) patients. Conclusion: While the majority of malignancies were found to have a low risk of containing malignant cells in ovarian tissue, further studies are needed to ensure safe implementation of future fertility restoration in clinical practice.

摘要翻译： 

背景：卵巢组织冷冻保存与移植是针对患有癌症的青春期前女孩唯一可行的生育力保存技术。尽管自体移植存在重新引入恶性细胞的风险，但通过识别卵巢组织中的微小浸润性疾病（MID）可避免此风险。方法：根据PRISMA指南，对截至2023年3月PubMed数据库中的同行评审文献进行了广泛检索。检索词包括“微小残留病”、“冷冻保存”、“卵巢”、“癌症”及其同义词。结果：在542条已识别记录中，纳入了17篇文献。共评估了至少115名女孩的卵巢组织，并将其分类为：血液系统恶性肿瘤（n=56；48.7%）、实体肿瘤（n=42；36.5%）和中枢神经系统肿瘤（n=17；14.8%）。在25名患者（21.7%）的卵巢组织中，通过RT-qPCR、FISH或多色流式细胞术检测到MID：其中16例（64%）为急性淋巴细胞白血病（伴或不伴TRG的IgH重排、BCL-ABL1、EA2-PBX1、TEL-AML1融合转录本），3例（12%）为尤文肉瘤（EWS-FLI1融合转录本、EWSR1重排），3例（12%）为慢性粒细胞白血病（BCR-ABL1融合转录本、FLT3），3例（12%）为急性髓系白血病（白血病相关免疫表型、BCR-ABL1融合转录本）。结论：虽然大多数恶性肿瘤在卵巢组织中存在恶性细胞的风险较低，但仍需进一步研究以确保未来生育力恢复在临床实践中的安全实施。

原文链接：

Minimal Infiltrative Disease Identification in Cryopreserved Ovarian Tissue of Girls with Cancer for Future Use: A Systematic Review