文章：

揭示EV来源的miR-150-5p在前列腺癌转移中的作用及其与高格里森评分的关联：对诊断的启示

Unraveling the Role of EV-Derived miR-150-5p in Prostate Cancer Metastasis and Its Association with High-Grade Gleason Scores: Implications for Diagnosis

原文发布日期：17 August 2023

DOI: 10.3390/cancers15164148

类型: Article

开放获取: 是

英文摘要：

Metastasis remains the leading cause of mortality in prostate cancer patients. The presence of tumor cells in lymph nodes is an established prognostic indicator for several cancer types, such as melanoma, breast, oral, pancreatic, and cervical cancers. Emerging evidence highlights the role of microRNAs enclosed within extracellular vesicles as facilitators of molecular communication between tumors and metastatic sites in the lymph nodes. This study aims to investigate the potential diagnostic utility of EV-derived microRNAs in liquid biopsies for prostate cancer. By employing microarrays on paraffin-embedded samples, we characterized the microRNA expression profiles in metastatic lymph nodes, non-metastatic lymph nodes, and primary tumor tissues of prostate cancer. Differential expression of microRNAs was observed in metastatic lymph nodes compared to prostate tumors and non-metastatic lymph node tissues. Three microRNAs (miR-140-3p, miR-150-5p, and miR-23b-3p) were identified as differentially expressed between tissue and plasma samples. Furthermore, we evaluated the expression of these microRNAs in exosomes derived from prostate cancer cells and plasma samples. Intriguingly, high Gleason score samples exhibited the lowest expression of miR-150-5p compared to control samples. Pathway analysis suggested a potential regulatory role for miR-150-5p in the Wnt pathway and bone metastasis. Our findings suggest EV-derived miR-150-5p as a promising diagnostic marker for identifying patients with high-grade Gleason scores and detecting metastasis at an early stage.

摘要翻译： 

转移仍是前列腺癌患者死亡的主要原因。淋巴结中肿瘤细胞的存在是多种癌症（如黑色素瘤、乳腺癌、口腔癌、胰腺癌和宫颈癌）公认的预后指标。新近证据表明，细胞外囊泡包裹的微小RNA在肿瘤与淋巴结转移部位之间的分子通讯中发挥重要作用。本研究旨在探讨液体活检中EV来源的微小RNA在前列腺癌中的潜在诊断价值。通过对石蜡包埋样本进行微阵列分析，我们系统表征了前列腺癌转移淋巴结、非转移淋巴结及原发肿瘤组织中的微小RNA表达谱。与前列腺肿瘤和非转移淋巴结组织相比，转移淋巴结中观察到微小RNA的差异表达。其中三个微小RNA（miR-140-3p、miR-150-5p和miR-23b-3p）在组织样本与血浆样本间呈现表达差异。进一步评估发现，这些微小RNA在前列腺癌细胞来源的外泌体及血浆样本中均存在表达。值得注意的是，与对照组相比，高格里森评分样本中miR-150-5p表达水平最低。通路分析提示miR-150-5p可能在Wnt通路和骨转移过程中发挥调控作用。我们的研究结果表明，EV来源的miR-150-5p可作为识别高格里森评分患者及早期检测转移的潜在诊断标志物。

原文链接：

Unraveling the Role of EV-Derived miR-150-5p in Prostate Cancer Metastasis and Its Association with High-Grade Gleason Scores: Implications for Diagnosis