文章：

一种用于在种族/民族多样化人群中检测前列腺癌的抗肿瘤相关抗原自身抗体组合的Luminex方法

A Luminex Approach to Develop an Anti-Tumor-Associated Antigen Autoantibody Panel for the Detection of Prostate Cancer in Racially/Ethnically Diverse Populations

原文发布日期：11 August 2023

DOI: 10.3390/cancers15164064

类型: Article

开放获取: 是

英文摘要：

(1) Background: Autoantibodies to tumor-associated antigens (TAAs) have emerged as promising cancer biomarkers. Luminex technology offers a powerful approach for the simultaneous detection of multiple anti-TAA autoantibodies. (2) Methods: We aimed to utilize Luminex technology to evaluate and optimize a panel of anti-TAAs autoantibodies for detecting prostate cancer (PCa), which included autoantibodies to fourteen TAAs. A total of 163 serum samples (91 PCa, 72 normal controls) were screened to determine the levels of the autoantibodies using the Luminex assay. (3) Results: Twelve autoantibodies exhibited significantly high frequencies ranging from 19.8% to 51.6% in the PCa group. Receiver operating characteristic (ROC) curve analysis revealed area under the curve (AUC) values ranging from 0.609 to 0.868 for the twelve autoantibodies individually. We further confirmed the performance of the HSP60 autoantibody by using an enzyme-linked immunosorbent assay (ELISA) in a larger sample comprising 200 PCa sera, 20 benign prostatic hyperplasia (BPH) sera, and 137 normal control sera. The results obtained from the Luminex assay were consistent with the ELISA findings. We developed a panel consisting of three autoantibodies (p16, IMP2, and HSP60) which achieved an impressive AUC of 0.910 with a sensitivity of 71.4% and a specificity of 95.8%. The panel was also evaluated in PCa patients from different races/ethnicities with the best performance observed in distinguishing the Hispanic American patients with PCa from normal controls. (4) Conclusions: We developed an anti-TAA autoantibody panel for the detection of PCa that exhibits promising performance. This panel holds significant potential as a high-throughput tool to facilitate PCa detection.

摘要翻译： 

(1) 背景：针对肿瘤相关抗原的自身抗体已成为有前景的癌症生物标志物。Luminex技术为同步检测多种抗肿瘤相关抗原自身抗体提供了高效方法。  
(2) 方法：我们旨在运用Luminex技术评估并优化一组用于检测前列腺癌的抗肿瘤相关抗原自身抗体组合，共包含针对14种肿瘤相关抗原的自身抗体。通过Luminex检测法对163份血清样本进行筛查，以确定自身抗体水平。  
(3) 结果：在前列腺癌组中，12种自身抗体显示出显著高阳性频率。受试者工作特征曲线分析显示，这12种自身抗体的曲线下面积值为0.609至0.868。我们进一步通过酶联免疫吸附测定法验证了HSP60自身抗体的性能。Luminex检测结果与酶联免疫吸附测定结果一致。我们构建了包含三种自身抗体的组合，该组合展现出优异的诊断性能。该组合在不同种族/族裔的前列腺癌患者中均表现出良好的鉴别能力。  
(4) 结论：我们开发了一种用于检测前列腺癌的抗肿瘤相关抗原自身抗体组合，该组合表现出良好的性能，有望成为促进前列腺癌筛查的高通量工具。

原文链接：

A Luminex Approach to Develop an Anti-Tumor-Associated Antigen Autoantibody Panel for the Detection of Prostate Cancer in Racially/Ethnically Diverse Populations