Background: Prostate cancer is the second most common cancer in males worldwide, with αVβ5 in-tegrin, a coactivator receptor, being highly expressed in advanced prostate cancer. Irisin, a hormone secreted from skeletal muscles, can reduce cell viability and migration and potentially inhibit αVβ5. Objective: This study investigates the potential impact of irisin on prostate cancer cells and its underlying mechanism. Methods: In vitro evaluation of the antiproliferative action of irisin on metastatic prostate cancer (PC-3) cells was tested through MTT assay, flow cytometry, and Western blot. An in vivo evaluation of the antiproliferative effect on prostate cancer xenograft was evaluated in nude mice. Results: In vitro evaluations showed that irisin reduced PC-3 cell viability to 70% and increased the Annexin-V/7AAD positive cell population. Irisin altered the expression of apoptotic proteins, αVβ5, and proteins involved in the P13k-Akt pathway. In vivo, irisin inhibited tumor growth and progression, positively affecting animal well-being. In conclusion, irisin has an apoptotic effect on PC-3, possibly through altering αVβ5 and the Bcl2/BAX and P13k-Akt signaling pathway, inhibiting tumor growth in vivo. Conclusion: Our findings can serve as a foundation for further evaluation of irisin’s role in prostate cancer.
背景：前列腺癌是全球男性第二大常见癌症，其中共激活受体αVβ5整合素在晚期前列腺癌中高表达。鸢尾素是一种由骨骼肌分泌的激素，能降低细胞活力与迁移能力，并可能抑制αVβ5整合素。目的：本研究探讨鸢尾素对前列腺癌细胞的潜在作用及其机制。方法：通过MTT检测、流式细胞术和Western blot在体外评估鸢尾素对转移性前列腺癌（PC-3）细胞的抗增殖作用，并在裸鼠体内通过前列腺癌异种移植模型评估其抗增殖效应。结果：体外实验显示鸢尾素将PC-3细胞存活率降低至70%，并增加Annexin-V/7AAD阳性细胞群。鸢尾素可改变凋亡相关蛋白、αVβ5整合素及P13k-Akt通路蛋白的表达。在体内实验中，鸢尾素抑制肿瘤生长与进展，并对动物状态产生积极影响。结论：鸢尾素可能通过调控αVβ5整合素及Bcl2/BAX与P13k-Akt信号通路，对PC-3细胞产生促凋亡作用，并在体内抑制肿瘤生长。本研究可为深入探讨鸢尾素在前列腺癌中的作用奠定基础。
Irisin Induces Apoptosis in Metastatic Prostate Cancer Cells and Inhibits Tumor Growth In Vivo
肿瘤(癌症)患者之家