文章：

肿瘤生物学中的多重蛋白成像

Multiplex protein imaging in tumour biology

原文发布日期：2024-02-05

DOI: 10.1038/s41568-023-00657-4

类型: Review Article

开放获取: 否

英文摘要：

Tissue imaging has become much more colourful in the past decade. Advances in both experimental and analytical methods now make it possible to image protein markers in tissue samples in high multiplex. The ability to routinely image 40–50 markers simultaneously, at single-cell or subcellular resolution, has opened up new vistas in the study of tumour biology. Cellular phenotypes, interaction, communication and spatial organization have become amenable to molecular-level analysis, and application to patient cohorts has identified clinically relevant cellular and tissue features in several cancer types. Here, we review the use of multiplex protein imaging methods to study tumour biology, discuss ongoing attempts to combine these approaches with other forms of spatial omics, and highlight challenges in the field.

摘要翻译： 

组织成像在过去十年中变得丰富多彩。实验和分析方法的进步使得对组织样本中蛋白质标记进行高度多重成像成为可能。能够常规同时成像40-50种标记，并以单细胞或亚细胞分辨率进行观察，为肿瘤生物学研究开辟了新视野。细胞表型、相互作用、通讯和空间组织已可进行分子水平分析，通过对患者队列的应用，已在多种癌症类型中识别出具有临床意义的细胞和组织特征。本文回顾了多重蛋白质成像方法在肿瘤生物学研究中的应用，讨论了将这些方法与其它空间组学形式相结合的持续尝试，并重点指出了该领域面临的挑战。

原文链接：

Multiplex protein imaging in tumour biology