文章：

应用临床前模型和方法研究解剖转移

Dissecting metastasis using preclinical models and methods

原文发布日期：2023-05-03

DOI: 10.1038/s41568-023-00568-4

类型: Review Article

开放获取: 否

英文摘要：

Metastasis has long been understood to lead to the overwhelming majority of cancer-related deaths. However, our understanding of the metastatic process, and thus our ability to prevent or eliminate metastases, remains frustratingly limited. This is largely due to the complexity of metastasis, which is a multistep process that likely differs across cancer types and is greatly influenced by many aspects of the in vivo microenvironment. In this Review, we discuss the key variables to consider when designing assays to study metastasis: which source of metastatic cancer cells to use and where to introduce them into mice to address different questions of metastasis biology. We also examine methods that are being used to interrogate specific steps of the metastatic cascade in mouse models, as well as emerging techniques that may shed new light on previously inscrutable aspects of metastasis. Finally, we explore approaches for developing and using anti-metastatic therapies, and how mouse models can be used to test them.

摘要翻译： 

长期以来，转移被认为是导致绝大多数癌症相关死亡的原因。然而，我们对转移过程的理解以及预防或消除转移的能力仍然有限，这令人沮丧。这很大程度上是由于转移的复杂性，它是一个多步骤过程，可能因癌症类型而异，并且受到体内微环境许多方面的极大影响。在本综述中，我们讨论了设计研究转移的实验时需要考虑的关键变量：使用哪种转移性癌细胞来源以及将其引入小鼠体内的哪个部位，以解决转移生物学的不同问题。我们还考察了用于在小鼠模型中研究转移级联特定步骤的方法，以及可能揭示转移以前难以理解方面的新兴技术。最后，我们探讨了开发和使用抗转移疗法的方法，以及如何利用小鼠模型来测试这些疗法。

原文链接：

Dissecting metastasis using preclinical models and methods