文章：

通过循环肿瘤细胞的单细胞谱分析揭示肿瘤异质性

Unravelling tumour heterogeneity by single-cell profiling of circulating tumour cells

原文发布日期：2019-08-27

DOI: 10.1038/s41568-019-0180-2

类型: Review Article

开放获取: 否

英文摘要：

Single-cell technologies have contributed to unravelling tumour heterogeneity, now considered a hallmark of cancer and one of the main causes of tumour resistance to cancer therapies. Liquid biopsy (LB), defined as the detection and analysis of cells or cell products released by tumours into the blood, offers an appealing minimally invasive approach that allows the characterization and monitoring of tumour heterogeneity in individual patients. Here, we will review and discuss how circulating tumour cell (CTC) analysis at single-cell resolution provides unique insights into tumour heterogeneity that are not revealed by analysis of circulating tumour DNA (ctDNA) derived from LBs. The molecular analysis of CTCs provides complementary information to that of genomic aberrations determined using ctDNA to fully describe many different cellular components (for example, DNA, RNA, proteins and metabolites) that can influence tumour heterogeneity.

摘要翻译： 

单细胞技术助力揭示肿瘤异质性——当前学界将其视为癌症的重要特征及肿瘤抵抗治疗的主要原因之一。液体活检作为一种通过检测和分析肿瘤释放到血液中的细胞或细胞产物的微创检测手段，为个体化表征和监测肿瘤异质性提供了颇具吸引力的解决方案。本文将重点评述循环肿瘤细胞单细胞分辨率分析如何揭示液体活检中循环肿瘤DNA分析无法展现的肿瘤异质性独特视角。CTC分子分析能够与ctDNA检测的基因组异常信息形成互补，从而全面解析影响肿瘤异质性的多种细胞成分（如DNA、RNA、蛋白质及代谢产物）。

原文链接：

Unravelling tumour heterogeneity by single-cell profiling of circulating tumour cells