文章：

ProHNPs是正常骨髓形成的特异性标志物

ProHNPs are specific markers of normal myelopoiesis

原文发布日期：2014-03-21

DOI: 10.1038/bcj.2014.11

类型: Original Article

开放获取: 是

英文摘要：

Pro human neutrophil peptides (proHNP)s are proforms of α-defensins produced by precursors of human neutrophils. They are secreted to bone marrow plasma in large amounts by myelocytes. We hypothesized that the plasma concentration of proHNPs might serve as a specific marker of myelopoietic activity, heralding the onset of normal myelopoiesis before reappearance of neutrophils, in the setting of bone marrow regeneration. To investigate this, plasma levels of proHNPs were measured by enzyme-linked immunosorbent assay in blood samples collected from patients undergoing allogeneic (n=11) or autologous (n=16) stem cell transplantations (SCTs) and patients receiving chemotherapy for acute leukemia (n=14). To compare proHNPs with previously suggested myeloid markers, myeloperoxidase (MPO), lysozyme and neutrophil gelatinase-associated lipocalin (NGAL) were also assayed. In all but one patient, chemotherapy led to the complete disappearance of ProHNPs from plasma. It reappeared in plasma on average 6.3 days before reappearance of neutrophils in the allogeneic setting, whereas this was reduced to an average of 2.8 days in the autologous SCT patients who received granulocyte colony-stimulating factor. Patients with acute myeloid leukemia (n=19) had significantly lower levels of plasma proHNPs than healthy controls, indicating that proHNPs are not produced by leukemic blasts. We conclude that plasma concentration of proHNPs is a clinically useful marker of normal myelopoiesis.

摘要翻译： 

人中性粒细胞前肽（proHNP）是中性粒细胞前体产生的α-防御素前体形式，由髓细胞大量分泌至骨髓血浆。我们提出假设：在骨髓再生过程中，proHNP的血浆浓度可作为髓系造血活性的特异性标志物，预示中性粒细胞重现前的正常髓系造血启动。为验证此假设，我们通过酶联免疫吸附法检测了接受异体（n=11）或自体（n=16）干细胞移植患者及急性白血病化疗患者（n=14）血样中的proHNP血浆水平。为与既往提出的髓系标志物对比，同时检测了髓过氧化物酶（MPO）、溶菌酶和中性粒细胞明胶酶相关脂质运载蛋白（NGAL）。除一例患者外，化疗均导致proHNP从血浆中完全消失。在异体移植中，proHNP平均早于中性粒细胞6.3天重现血浆；而接受粒细胞集落刺激因子的自体移植患者中，该间隔缩短至平均2.8天。急性髓系白血病患者（n=19）血浆proHNP水平显著低于健康对照组，表明白血病原始细胞不产生proHNP。我们得出结论：血浆proHNP浓度是临床评估正常髓系造血的有效标志物。

原文链接：

ProHNPs are specific markers of normal myelopoiesis