文章：

八色流式细胞术监测白血病相关免疫表型在成人b急性淋巴细胞白血病中的预后意义

Prognostic significance of monitoring leukemia-associated immunophenotypes by eight-color flow cytometry in adult B-acute lymphoblastic leukemia

原文发布日期：2013-08-16

DOI: 10.1038/bcj.2013.31

类型: Original Article

开放获取: 是

英文摘要：

Minimal residual disease (MRD) is of the most important factor for predicting prognosis and guiding treatment of acute lymphoblastic leukemia (ALL). In this study, we investigated the prognostic significance of leukemia-associated immunophenotypes (LAIPs) as assessment of index of MRD in 125 adult B-lineage ALL (B-ALL) patients by eight-color flow cytometry. The LAIPs could be identified in 96% and 81.6% of patients with the sensitivity of 10−4 and 10−5, respectively. MRD-negative status could clearly predict a favorable 2-year relapse-free survival (RFS) and overall survival (OS) at the end of induction of complete remission and one cycle of consolidation treatment. Moreover, we identified a group of cases with MRD of 0.001% to <0.01%, which showed significantly higher 2-year relapse rate than those with undetectable one. In multivariate analysis, MRD status was associated with RFS or OS independently. Furthermore, MRD assessed by LAIPs and RQ-PCR assay for patients with BCR-ABL fusion gene yielded concordant results in 89.7% of cases. In conclusion, MRD evaluated by eight-color flow cytometry could provide an important tool to assess treatment response and prognosis precisely in adult B-ALL.

摘要翻译： 

微小残留病（MRD）是预测急性淋巴细胞白血病（ALL）预后和指导治疗的最重要因素。本研究通过八色流式细胞术检测125例成人B系急性淋巴细胞白血病（B-ALL）患者白血病相关免疫表型（LAIPs），以评估MRD指标的预后意义。在检测灵敏度为10^-4和10^-5时，分别有96%和81.6%的患者可识别出LAIPs。达到完全缓解诱导治疗结束及一个周期巩固治疗后的MRD阴性状态，可明确预测良好的2年无复发生存期（RFS）和总生存期（OS）。此外，我们发现MRD水平在0.001%至<0.01%的病例组，其2年复发率显著高于MRD不可测组。多变量分析显示，MRD状态与RFS或OS独立相关。进一步对携带BCR-ABL融合基因的患者采用LAIPs与实时定量PCR（RQ-PCR）检测MRD，结果显示89.7%的病例检测结果一致。综上所述，通过八色流式细胞术评估MRD可为精准判断成人B-ALL治疗反应及预后提供重要工具。

原文链接：

Prognostic significance of monitoring leukemia-associated immunophenotypes by eight-color flow cytometry in adult B-acute lymphoblastic leukemia